دورية أكاديمية
[Determination of Antimony Resistance Mechanism of Leishmania tropica Causing Cutaneous Leishmaniasis in Turkey].
| العنوان: | [Determination of Antimony Resistance Mechanism of Leishmania tropica Causing Cutaneous Leishmaniasis in Turkey]. |
|---|---|
| عنوان ترانسليتريتد: | Türkiye’de Kutanöz Leyşmanyazis Etkeni Leishmania tropica’da Antimon Direnç Mekanizmasının Belirlenmesi. |
| المؤلفون: | Özbilgin A; Manisa Celal Bayar University Faculty of Medicine, Department of Medical Parasitology, Manisa, Turkey., Zeyrek FY; Harran University Faculty of Medicine, Department of Medical Microbiology, Sanliurfa, Turkey., Güray MZ; Izmir Institute of High Technology, Department of Chemistry, Izmir, Turkey., Çulha G; Mustafa Kemal University Faculty of Medicine, Department of Parasitology, Hatay, Turkey., Akyar I; Acibadem University Faculty of Medicine, Department of Medical Microbiology, Istanbul, Turkey., Harman M; Dicle University Faculty of Medicine, Department of Dermatology, Diyarbakir, Turkey., Özbel Y; Ege University Faculty of Medicine, Department of Medical Parasitology, Izmir, Turkey., Ertabaklar H; Adnan Menderes University Faculty of Medicine, Department of Medical Parasitology, Aydin, Turkey., Çavuş İ; Manisa Celal Bayar University Faculty of Medicine, Department of Medical Parasitology, Manisa, Turkey., Gündüz C; Ege University Faculty of Medicine, Department of Medical Biology, Izmir, Turkey. |
| المصدر: | Mikrobiyoloji bulteni [Mikrobiyol Bul] 2020 Jul; Vol. 54 (3), pp. 444-462. |
| نوع المنشور: | Journal Article |
| اللغة: | Turkish |
| بيانات الدورية: | Publisher: Ankara Mikrobiyoloji Dernegi Country of Publication: Turkey NLM ID: 7503830 Publication Model: Print Cited Medium: Print ISSN: 0374-9096 (Print) Linking ISSN: 03749096 NLM ISO Abbreviation: Mikrobiyol Bul Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Ankara : Ankara Mikrobiyoloji Dernegi Original Publication: Ankara. |
| مواضيع طبية MeSH: | Drug Resistance*/genetics , Leishmania tropica*/drug effects , Leishmaniasis, Cutaneous*/drug therapy , Meglumine Antimoniate*/pharmacology , Meglumine Antimoniate*/therapeutic use, Humans ; Turkey |
| مستخلص: | World Health Organization reported that approximately one billion people are at risk in endemic areas, one million cases of cutaneous leishmaniasis (CL) and approximately 300,000 cases of visceral leishmaniasis (VL) were reported per year in the last five years. The number of deaths due to VL is reported to be approximately 20,000 per year. Approximately 2500 cases/year have been reported as CL, caused by Leishmania tropica and Leishmania infantum, in Turkey. The significant increase observed in many cities mainly in the provinces of Mediterranean and Aegean regions in cases and foci in recent years, suggests that there may be an increase in this infections in the following years as well. In Turkey, the causative agent of CL is L.tropica and meglumine antimoniate is used in the treatment of CL. We aimed to determine antimony resistance genes specific for L.tropica by comparing the gene and protein expressions of antimony-resistant and non-resistant L.tropica strains. L.tropica isolates obtained from 3 CL patients without antimonate resistance from Aegean, Mediterranean and Southeastern regions of Turkey were provided to transform into 3 resistant isolates against meglumine antimony in the laboratory conditions. Gene expression alterations by microarray method; protein profiles by two-dimensional gel electrophoresis (2D-PAGE) and relevant proteins by MALDI-TOF/TOF MS of these isolates were accomplished and compared. L.tropica isolates from 10 CL patients who did not respond to antimony therapy were analyzed for resistance to antimonial compounds and quantitative real-time polymerase chain reaction was performed to detect the expression of genes responsible for resistance development. Moreover, differences in protein expression levels in isolates with and without antimony resistance were determined by comparing protein profiles and identification of proteins with different expression levels was carried out. Enolase, elongation factor-2, heat shock protein 70, tripanthione reductase, protein kinase C and metallo-peptidase proteins have been shown to play roles in L.tropica isolates developing resistance to antimonial compounds and similar expression changes have also been demonstrated in naturally resistant isolates from patients. In conclusion, it was revealed that L.tropica strains in our country may gain resistance to meglumine antimoniate in a short time. It is foreseen that if the patients living in our country or entering the country are treated inadequately and incompletely, there may be new, resistant leishmaniasis foci that may increase the number of resistant strains and cases rapidly. |
| المشرفين على المادة: | 75G4TW236W (Meglumine Antimoniate) |
| تواريخ الأحداث: | Date Created: 20200807 Date Completed: 20200818 Latest Revision: 20200818 |
| رمز التحديث: | 20221213 |
| DOI: | 10.5578/mb.69702 |
| PMID: | 32755520 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0374-9096 |
|---|---|
| DOI: | 10.5578/mb.69702 |