دورية أكاديمية
Molecular profiling of non-small cell lung cancer.
العنوان: | Molecular profiling of non-small cell lung cancer. |
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المؤلفون: | Forsythe ML; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada., Alwithenani A; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada.; Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University Makkah, Makkah, Saudi Arabia., Bethune D; Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Castonguay M; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada., Drucker A; Division of Medical Oncology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Flowerdew G; Department of Epidemiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., French D; Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Fris J; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada., Greer W; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada., Henteleff H; Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., MacNeil M; Division of Medical Oncology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Marignani P; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Morzycki W; Division of Medical Oncology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Plourde M; Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Snow S; Division of Medical Oncology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada., Xu Z; Department of Pathology, Faculty of Medicine, Dalhousie University Halifax, Halifax, Nova Scotia, Canada. |
المصدر: | PloS one [PLoS One] 2020 Aug 05; Vol. 15 (8), pp. e0236580. Date of Electronic Publication: 2020 Aug 05 (Print Publication: 2020). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Genetic Predisposition to Disease* , Prognosis*, Adenocarcinoma/*genetics , Carcinoma, Non-Small-Cell Lung/*genetics, Adenocarcinoma/classification ; Adenocarcinoma/epidemiology ; Adenocarcinoma/pathology ; Adult ; Aged ; Aged, 80 and over ; Anaplastic Lymphoma Kinase/genetics ; Carcinoma, Non-Small-Cell Lung/classification ; Carcinoma, Non-Small-Cell Lung/epidemiology ; Carcinoma, Non-Small-Cell Lung/pathology ; Class I Phosphatidylinositol 3-Kinases/genetics ; ErbB Receptors/genetics ; Female ; Humans ; Male ; Middle Aged ; Mutation/genetics ; Proto-Oncogene Proteins B-raf/genetics ; Proto-Oncogene Proteins p21(ras)/genetics ; Receptor, ErbB-2/genetics |
مستخلص: | Lung cancer is generally treated with conventional therapies, including chemotherapy and radiation. These methods, however, are not specific to cancer cells and instead attack every cell present, including normal cells. Personalized therapies provide more efficient treatment options as they target the individual's genetic makeup. The goal of this study was to identify the frequency of causal genetic mutations across a variety of lung cancer subtypes in the earlier stages. 833 samples of non-small cell lung cancer from 799 patients who received resection of their lung cancer, were selected for molecular analysis of six known mutations, including EGFR, KRAS, BRAF, PIK3CA, HER2 and ALK. A SNaPshot assay was used for point mutations and fragment analysis searched for insertions and deletions. ALK was evaluated by IHC +/- FISH. Statistical analysis was performed to determine correlations between molecular and clinical/pathological patient data. None of the tested variants were identified in most (66.15%) of cases. The observed frequencies among the total samples vs. only the adenocarcinoma cases were notable different, with the highest frequency being the KRAS mutation (24.49% vs. 35.55%), followed by EGFR (6.96% vs. 10.23%), PIK3CA (1.20% vs. 0.9%), BRAF (1.08% vs. 1.62%), ALK (0.12% vs. 0.18%), while the lowest was the HER2 mutation (0% for both). The statistical analysis yielded correlations between presence of a mutation with gender, cancer type, vascular invasion and smoking history. The outcome of this study will provide data that helps stratify patient prognosis and supports development of more precise treatments, resulting in improved outcomes for future lung cancer patients. Competing Interests: The study was partially supported by Roche Canada, Pfizer Canada and Boehringer Ingelheim Canada. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funding provided by the pharmaceutical companies were helping us for validation of the tests and not related to employment, consultancy, patents, products in development, marketed products, and any commercial purposes. This does not alter our adherence to PLOS ONE policies on sharing data and materials. |
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المشرفين على المادة: | 0 (KRAS protein, human) EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases) EC 2.7.1.137 (PIK3CA protein, human) EC 2.7.10.1 (ALK protein, human) EC 2.7.10.1 (Anaplastic Lymphoma Kinase) EC 2.7.10.1 (EGFR protein, human) EC 2.7.10.1 (ERBB2 protein, human) EC 2.7.10.1 (ErbB Receptors) EC 2.7.10.1 (Receptor, ErbB-2) EC 2.7.11.1 (BRAF protein, human) EC 2.7.11.1 (Proto-Oncogene Proteins B-raf) EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras)) |
تواريخ الأحداث: | Date Created: 20200807 Date Completed: 20201002 Latest Revision: 20201002 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC7406040 |
DOI: | 10.1371/journal.pone.0236580 |
PMID: | 32756609 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
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DOI: | 10.1371/journal.pone.0236580 |