دورية أكاديمية
Influence of Microwave-Assisted Drying on Structural Integrity and Viability of Testicular Tissues from Adult and Prepubertal Domestic Cats.
| العنوان: | Influence of Microwave-Assisted Drying on Structural Integrity and Viability of Testicular Tissues from Adult and Prepubertal Domestic Cats. |
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| المؤلفون: | Silva HVR; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, Brazil., da Silva AM; Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, Brazil., Lee PC; Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, USA., Brito BF; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, Brazil., Silva AR; Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, Brazil., da Silva LDM; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, Brazil., Comizzoli P; Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, USA. |
| المصدر: | Biopreservation and biobanking [Biopreserv Biobank] 2020 Oct; Vol. 18 (5), pp. 415-424. Date of Electronic Publication: 2020 Aug 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 101507284 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1947-5543 (Electronic) Linking ISSN: 19475543 NLM ISO Abbreviation: Biopreserv Biobank Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New Rochelle, NY : Mary Ann Liebert |
| مواضيع طبية MeSH: | Microwaves*, Animals ; Cats ; Desiccation ; Preservation, Biological ; Temperature ; Trehalose ; Water |
| مستخلص: | Anhydrous preservation is a promising approach for storage of living biomaterials at nonfreezing temperatures. Using the domestic cat model, the objectives of this study were to characterize changes in histology, DNA integrity, and viability of testicular tissues from adult versus prepubertal individuals during microwave-assisted drying. Testes from each age group were cut into small pieces before reversible membrane permeabilization, exposure to trehalose, and microwave-assisted drying during different time periods. In Experiment 1, water content was monitored for up to 40 minutes of drying. Tissues from adult or prepubertal cats experienced similar decreases of water content during the first 10 minutes. Desiccation progressed slowly between 10 and 20 minutes and then remained stable. In Experiment 2, structural properties were explored at 5, 10, and 20 minutes of desiccation. Percentages of normal seminiferous tubules were lower after 20 minutes drying in adult (43%) than in prepubertal tissues (61%). At the same time point, the proportion of cell degeneration was higher in adult (53%) than prepubertal tissues (28%). Percentages of intact DNA in tissues remained above 85% regardless of the microwave time in both age groups. Lastly, adult and prepubertal tissues only lost 33% of viability in both age groups. Collective results demonstrated for the first time that normal morphology, incidence of degeneration, DNA integrity, and viability of testicular tissues remained at acceptable levels during microwave-assisted drying for 20 minutes. Overall, prepubertal testicular tissues appeared to be more resilient to microwave-assisted desiccations than adult tissues. Importantly, water loss in the presence of trehalose after 20 minutes of desiccation already is compatible with long-term storage of testicular tissues at temperatures above -20°C, which is one step closer to future storage at supra-zero temperatures. |
| فهرسة مساهمة: | Keywords: drying; fertility preservation; testicular tissue |
| المشرفين على المادة: | 059QF0KO0R (Water) B8WCK70T7I (Trehalose) |
| تواريخ الأحداث: | Date Created: 20200812 Date Completed: 20210902 Latest Revision: 20210902 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1089/bio.2020.0048 |
| PMID: | 32780644 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1947-5543 |
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| DOI: | 10.1089/bio.2020.0048 |