دورية أكاديمية
Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia.
العنوان: | Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia. |
---|---|
المؤلفون: | Mietus CJ; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Lackner TJ; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Karvelis PS; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Willcockson GT; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Shields CM; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Lambert NG; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Koutakis P; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA.; Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, FL 32306, USA., Fuglestad MA; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Hernandez H; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., Haynatzki GR; Department of Biostatistics, University of Nebraska Medical Center, Omaha, NE 68198-4375, USA., Kim JKS; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA., DeSpiegelaere HK; Department of Surgery and VA Research Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE 68105-1873, USA., Pipinos II; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA.; Department of Surgery and VA Research Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE 68105-1873, USA., Casale GP; Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA. |
المصدر: | Journal of clinical medicine [J Clin Med] 2020 Aug 08; Vol. 9 (8). Date of Electronic Publication: 2020 Aug 08. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101606588 Publication Model: Electronic Cited Medium: Print ISSN: 2077-0383 (Print) Linking ISSN: 20770383 NLM ISO Abbreviation: J Clin Med Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2012]- |
مستخلص: | Work from our laboratory documents pathological events, including myofiber oxidative damage and degeneration, myofibrosis, micro-vessel (diameter = 50-150 μm) remodeling, and collagenous investment of terminal micro-vessels (diameter ≤ 15 µm) in the calf muscle of patients with Peripheral Artery Disease (PAD). In this study, we evaluate the hypothesis that the vascular pathology associated with the legs of PAD patients encompasses pathologic changes to the smallest micro-vessels in calf muscle. Biopsies were collected from the calf muscle of control subjects and patients with Fontaine Stage II and Stage IV PAD. Slide specimens were evaluated by Quantitative Multi-Spectral and Fluorescence Microscopy. Inter-myofiber collagen, stained with Masson Trichrome (MT), was increased in Stage II patients, and more substantially in Stage IV patients in association with collagenous thickening of terminal micro-vessel walls. Evaluation of the Basement Membrane (BM) of these vessels reveals increased thickness in Stage II patients, and increased thickness, diameter, and Collagen I deposition in Stage IV patients. Coverage of these micro-vessels with pericytes, key contributors to fibrosis and BM remodeling, was increased in Stage II patients, and was greatest in Stage IV patients. Vascular pathology of the legs of PAD patients extends beyond atherosclerotic main inflow arteries and affects the entire vascular tree-including the smallest micro-vessels. |
References: | Am J Physiol Regul Integr Comp Physiol. 2013 Aug 1;305(3):R291-9. (PMID: 23720135) J Surg Res. 2015 Jun 1;196(1):172-9. (PMID: 25791828) Echocardiography. 2017 Aug;34(8):1187-1194. (PMID: 28664576) Circ Res. 2015 Apr 24;116(9):1527-39. (PMID: 25908726) J Transl Med. 2013 Sep 25;11:230. (PMID: 24067235) J Cardiopulm Rehabil Prev. 2008 Nov-Dec;28(6):349-57. (PMID: 19008688) Vasc Med. 2018 Apr;23(2):143-152. (PMID: 29633922) Microsc Microanal. 2012 Feb;18(1):68-80. (PMID: 22166617) J Vasc Endovasc Surg. 2017;2(1):. (PMID: 28691118) Am J Physiol Cell Physiol. 2013 Dec 1;305(11):C1098-113. (PMID: 24067916) Circulation. 1998 Feb 10;97(5):473-83. (PMID: 9490243) Circulation. 2010 Nov 2;122(18):1862-75. (PMID: 21041698) Helv Chir Acta. 1954 Dec;21(5-6):499-533. (PMID: 14366554) J Vasc Surg. 2000 Jan;31(1 Pt 2):S1-S296. (PMID: 10666287) Elife. 2018 Mar 21;7:. (PMID: 29561727) Diabetes Obes Metab. 2002 Mar;4 Suppl 2:S1-6. (PMID: 12180352) Circ Res. 2015 Apr 24;116(9):1540-50. (PMID: 25908727) Eur Heart J. 2006 Feb;27(3):310-5. (PMID: 16308326) Med Biol Eng Comput. 2016 Nov;54(11):1667-1681. (PMID: 26906279) Stem Cell Res Ther. 2014 Nov 06;5(6):122. (PMID: 25376879) Am J Physiol Lung Cell Mol Physiol. 2009 Mar;296(3):L489-99. (PMID: 19036873) Am J Physiol Lung Cell Mol Physiol. 2006 Apr;290(4):L661-L673. (PMID: 16227320) Circulation. 2014 Apr 15;129(15):1586-97. (PMID: 24481949) Cardiovasc Diabetol. 2005 Jun 28;4:9. (PMID: 15985157) F1000Prime Rep. 2013 Sep 02;5:37. (PMID: 24049641) J Vasc Surg. 2007 Jan;45 Suppl S:S5-67. (PMID: 17223489) J Transl Med. 2016 Feb 04;14:39. (PMID: 26847457) Microcirculation. 2005 Sep;12(6):527-37. (PMID: 16147469) Vasc Endovascular Surg. 2007 Dec-2008 Jan;41(6):481-9. (PMID: 18166628) Front Physiol. 2017 Mar 13;8:141. (PMID: 28348531) Nat Med. 2012 Aug;18(8):1262-70. (PMID: 22842476) Diabetologia. 1985 Dec;28(12):895-900. (PMID: 4092858) Circulation. 1993 Feb;87(2):413-21. (PMID: 8425290) Redox Biol. 2014 Jul 19;2:921-8. (PMID: 25180168) Sci Rep. 2017 Aug 29;7(1):9815. (PMID: 28852062) Vasc Endovascular Surg. 2008 Apr-May;42(2):101-12. (PMID: 18390972) Am J Pathol. 2013 Jan;182(1):118-31. (PMID: 23142380) Invest Radiol. 2011 Aug;46(8):504-8. (PMID: 21487300) |
معلومات مُعتمدة: | R01 AG049868 United States AG NIA NIH HHS; R01 AG034995 and R01 AG049868 United States NH NIH HHS |
فهرسة مساهمة: | Keywords: basement membrane thickening; fibrosis; microvascular pathology; αSMA+ pericytes |
تواريخ الأحداث: | Date Created: 20200814 Latest Revision: 20210803 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC7464726 |
DOI: | 10.3390/jcm9082575 |
PMID: | 32784470 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2077-0383 |
---|---|
DOI: | 10.3390/jcm9082575 |