دورية أكاديمية
أعرض في EDS

Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism.

التفاصيل البيبلوغرافية
العنوان: Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism.
المؤلفون: Pchelin IM; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Mochalov YV; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Azarov DV; Department of Epidemiology, Parasitology and Disinfectology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Romanyuk SA; City Hospital No. 40, Saint Petersburg, Russia., Chilina GA; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Vybornova IV; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Bogdanova TV; Department of Medical Microbiology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Zlatogursky VV; Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Saint Petersburg, Russia., Apalko SV; City Hospital No. 40, Saint Petersburg, Russia., Vasilyeva NV; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia.; Department of Medical Microbiology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia., Taraskina AE; Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia.
المصدر: Mycoses [Mycoses] 2020 Nov; Vol. 63 (11), pp. 1244-1254. Date of Electronic Publication: 2020 Sep 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Grosse Verlag Country of Publication: Germany NLM ID: 8805008 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0507 (Electronic) Linking ISSN: 09337407 NLM ISO Abbreviation: Mycoses Subsets: PubMed not MEDLINE; MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Grosse Verlag, [1988?-
مستخلص: Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population.
Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure.
Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction.
Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5'-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity.
Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.
(© 2020 Wiley-VCH GmbH.)
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معلومات مُعتمدة: 18-34-00153 Russian Foundation for Basic Research
فهرسة مساهمة: Keywords: Trichophyton rubrum; dermatophyte; microsatellites; molecular strain typing; population structure; whole-genome sequencing
تواريخ الأحداث: Date Created: 20200814 Latest Revision: 20231129
رمز التحديث: 20240628
DOI: 10.1111/myc.13162
PMID: 32785975
قاعدة البيانات: MEDLINE
الوصف
تدمد:1439-0507
DOI:10.1111/myc.13162