دورية أكاديمية
An Improved Intracellular Synthetic Lipidation-Induced Plasma Membrane Anchoring System for SNAP-Tag Fusion Proteins.
| العنوان: | An Improved Intracellular Synthetic Lipidation-Induced Plasma Membrane Anchoring System for SNAP-Tag Fusion Proteins. |
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| المؤلفون: | Yoshii T; Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.; PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan., Tahara K; Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan., Suzuki S; Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan., Hatano Y; Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan., Kuwata K; Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan., Tsukiji S; Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.; Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan. |
| المصدر: | Biochemistry [Biochemistry] 2020 Aug 25; Vol. 59 (33), pp. 3044-3050. Date of Electronic Publication: 2020 Aug 15. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370623 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4995 (Electronic) Linking ISSN: 00062960 NLM ISO Abbreviation: Biochemistry Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Recombinant Fusion Proteins*/chemical synthesis , Recombinant Fusion Proteins*/chemistry , Recombinant Fusion Proteins*/metabolism, Cell Membrane/*metabolism , Lipid-Linked Proteins/*chemical synthesis , Membrane Lipids/*metabolism , Protein Engineering/*methods, Cell Membrane/chemistry ; Escherichia coli ; Lipid-Linked Proteins/chemistry ; Lipid-Linked Proteins/metabolism ; Membrane Lipids/chemistry ; Staining and Labeling/methods ; Tetrahydrofolate Dehydrogenase/chemistry ; Tetrahydrofolate Dehydrogenase/metabolism |
| مستخلص: | The ability to chemically introduce lipid modifications to specific intracellular protein targets would enable the conditional control of protein localization and activity in living cells. We recently developed a chemical-genetic approach in which an engineered SNAP-tag fusion protein can be rapidly relocated and anchored from the cytoplasm to the plasma membrane (PM) upon post-translational covalent lipopeptide conjugation in cells. However, the first-generation system achieved only low to moderate protein anchoring (recruiting) efficiencies and lacked wide applicability. Herein, we describe the rational design of an improved system for intracellular synthetic lipidation-induced PM anchoring of SNAP-tag fusion proteins. In the new system, the SNAP |
| المشرفين على المادة: | 0 (Lipid-Linked Proteins) 0 (Membrane Lipids) 0 (Recombinant Fusion Proteins) EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase) |
| تواريخ الأحداث: | Date Created: 20200814 Date Completed: 20210317 Latest Revision: 20210317 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acs.biochem.0c00410 |
| PMID: | 32786409 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-4995 |
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| DOI: | 10.1021/acs.biochem.0c00410 |