دورية أكاديمية
Toxicogenetic and antiproliferative effects of chrysin in urinary bladder cancer cells.
| العنوان: | Toxicogenetic and antiproliferative effects of chrysin in urinary bladder cancer cells. |
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| المؤلفون: | Lima APB; Programa de Pós-graduação em Ciências Farmacêuticas (CIPHARMA), Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brazil., Almeida TC; Programa de Pós-graduação em Ciências Farmacêuticas (CIPHARMA), Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brazil., Barros TMB; Programa de Pós-graduação em Ciências Farmacêuticas (CIPHARMA), Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brazil., Rocha LCM; Programa de Pós-graduação em Ciência Biológicas (CBIOL), Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, Minas Gerais, Brazil., Garcia CCM; Programa de Pós-graduação em Ciência Biológicas (CBIOL), Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, Minas Gerais, Brazil.; Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, Minas Gerais, Brazil., da Silva GN; Programa de Pós-graduação em Ciências Farmacêuticas (CIPHARMA), Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brazil.; Programa de Pós-graduação em Ciência Biológicas (CBIOL), Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, Minas Gerais, Brazil.; Departamento de Análises Clínicas (DEACL), Escola de Farmácia, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, Minas Gerais, Brazil. |
| المصدر: | Mutagenesis [Mutagenesis] 2020 Aug 13. Date of Electronic Publication: 2020 Aug 13. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8707812 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3804 (Electronic) Linking ISSN: 02678357 NLM ISO Abbreviation: Mutagenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1995- : Swansea, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the United Kingdom Environmental Mutagen Society by IRL Press, [1986- |
| مستخلص: | The antitumour activity of chrysin have been studied in several types of cancer cells. In urinary bladder cancer, its cytotoxic effects have already demonstrated; however, its mechanism of action is not completely understood and the role of tumour protein p53 (TP53) gene in these effects is unclear. In this study, we investigated the role of chrysin (10, 20, 40, 60 80 and 100 µM) in progression of bladder tumour cells with different status of the TP53 gene and different degrees of tumour (RT4, grade 1, TP53 wild type; 5637, grade 2, TP53 mutated and T24, grade 3, TP53 mutated). Results demonstrated that chrysin inhibited cell proliferation by increasing reactive oxygen species and DNA damage and inhibited cell migration in all cell lines. In TP53 wild-type cells, a sub-G1 apoptotic population was present. In mutated TP53 cells, chrysin caused arrest at the G2/M phase and morphological changes accompanied by downregulation of PLK1, SRC and HOXB3 genes. In addition, in Grade 2 cells, chrysin induced global DNA hypermethylation and, in the highest-grade cells, downregulated c-MYC, FGFR3 and mTOR gene expression. In conclusion, chrysin has antiproliferative and toxicogenetic activity in bladder tumour cells independently of TP53 status; however, the mechanisms of action are dependent on TP53 status. (© The Author(s) 2020. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| تواريخ الأحداث: | Date Created: 20200814 Latest Revision: 20240227 |
| رمز التحديث: | 20240227 |
| DOI: | 10.1093/mutage/geaa021 |
| PMID: | 32789469 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1464-3804 |
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| DOI: | 10.1093/mutage/geaa021 |