دورية أكاديمية
Validated Liquid Chromatography-Tandem Mass Spectroscopic Method for Simultaneous Determination of Certain Drugs Used in Cataract Surgery in Rabbit Aqueous Humor.
| العنوان: | Validated Liquid Chromatography-Tandem Mass Spectroscopic Method for Simultaneous Determination of Certain Drugs Used in Cataract Surgery in Rabbit Aqueous Humor. |
|---|---|
| المؤلفون: | Nassar MW; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt., Attia KA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt., Mohamed AA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.; Pharmaceutical Chemistry Department, Heliopolis University for Sustainable Development, 3 Belbis Disert Road, Alsalam City, Cairo 11777, Egypt., Said RA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.; Pharmaceutical Chemistry Department, Heliopolis University for Sustainable Development, 3 Belbis Disert Road, Alsalam City, Cairo 11777, Egypt., El-Olemy A; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt., Hasan MA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt. |
| المصدر: | Journal of chromatographic science [J Chromatogr Sci] 2020 Sep 29; Vol. 58 (9), pp. 814-822. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0173225 Publication Model: Print Cited Medium: Internet ISSN: 1945-239X (Electronic) Linking ISSN: 00219665 NLM ISO Abbreviation: J Chromatogr Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2012- > : Oxford : Oxford University Press Original Publication: Niles Il : Preston Technical Abstracts |
| مواضيع طبية MeSH: | Aqueous Humor/*chemistry , Chromatography, High Pressure Liquid/*methods , Ophthalmic Solutions/*analysis , Ophthalmic Solutions/*chemistry , Tandem Mass Spectrometry/*methods, Animals ; Cataract Extraction ; Cyclopentolate/analysis ; Female ; Fluoroquinolones/analysis ; Limit of Detection ; Linear Models ; Male ; Phenylephrine/analysis ; Rabbits ; Reproducibility of Results |
| مستخلص: | A sensitive, selective, accurate and precise ultra-high performance liquid chromatography-Tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous determination of drugs used as eye drops in cataract surgery in aqueous humor. Cataract surgery requires a powerful mydriatic eye drops combination such as cyclopentolate hydrochloride and phenylephrine hydrochloride to dilate the pupil and facilitate eye lens replacement and also requires strong fluoroquinolone antibiotic such as lomefloxacin hydrochloride. The method was performed with positive ion electrospray ionization and the analytes were quantified and monitored on a triple quadrupole mass spectrometer using multiple reaction monitoring scanning mode. Liquid-liquid extraction was used for the purification and preconcentration of analytes from rabbit aqueous humor matrix. Chromatographic elution was performed using an Phenomenex Luna® C18 (150 mm × 2.1 mm, 1.6 μm) column and moxifloxacin hydrochloride as internal standard with a mobile phase consisting of methanol:water:formic acid (70:29:1, by volume) at flow rate of 0.2 mL/min. Satisfactory results regarding linearity, recovery, stability, accuracy and precision of the analytes were obtained. Full validation of the procedure was performed according to the US Food and Drug Administration guidance for industry: bioanalytical method validation and European Medicines Agency (EMA) guideline on bioanalytical method validation. (© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.) |
| المشرفين على المادة: | 0 (Fluoroquinolones) 0 (Ophthalmic Solutions) 1WS297W6MV (Phenylephrine) I76F4SHP7J (Cyclopentolate) L6BR2WJD8V (lomefloxacin) |
| تواريخ الأحداث: | Date Created: 20200815 Date Completed: 20210416 Latest Revision: 20210416 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/chromsci/bmaa049 |
| PMID: | 32794561 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1945-239X |
|---|---|
| DOI: | 10.1093/chromsci/bmaa049 |