دورية أكاديمية
A Fungal Ascorbate Oxidase with Unexpected Laccase Activity.
| العنوان: | A Fungal Ascorbate Oxidase with Unexpected Laccase Activity. |
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| المؤلفون: | Braunschmid V; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria.; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria., Fuerst S; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria., Perz V; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria., Zitzenbacher S; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria., Hoyo J; Grup de Biotecnologia Molecular i Industrial, Department d'Enginyeria Química, Universitat Politècnica de Catalunya, Rambla Sant Nebridi, 08222 Terrassa, Spain., Fernandez-Sanchez C; Instituto de Microelectronica de Barcelona (IMB-CNM), CSIC, Campus UAB, 08193 Bellatera, Spain., Tzanov T; Grup de Biotecnologia Molecular i Industrial, Department d'Enginyeria Química, Universitat Politècnica de Catalunya, Rambla Sant Nebridi, 08222 Terrassa, Spain., Steinkellner G; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria.; Innophore GmbH, 8010 Graz, Austria., Gruber K; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria.; Structural Biology, Institute of Molecular Bioscience, University of Graz, 8010 Graz, Austria., Nyanhongo GS; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria.; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria., Ribitsch D; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria.; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria., Guebitz GM; Institute of Environmental Biotechnology, Department of Agrobiotechnology, University of Natural Resources and Life Sciences (BOKU), 3430 Tulln an der Donau, Austria.; Austrian Centre for Industrial Biotechnology (ACIB), 3430 Tulln an der Donau, Austria. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2020 Aug 11; Vol. 21 (16). Date of Electronic Publication: 2020 Aug 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Ascorbate Oxidase/*metabolism , Aspergillus flavus/*enzymology , Laccase/*metabolism, Amino Acid Sequence ; Ascorbate Oxidase/chemistry ; Copper/metabolism ; Kinetics ; Laccase/chemistry ; Models, Molecular ; Oxidation-Reduction ; Substrate Specificity |
| مستخلص: | Ascorbate oxidases are an enzyme group that has not been explored to a large extent. So far, mainly ascorbate oxidases from plants and only a few from fungi have been described. Although ascorbate oxidases belong to the well-studied enzyme family of multi-copper oxidases, their function is still unclear. In this study, Af _AO1, an enzyme from the fungus Aspergillus flavus , was characterized. Sequence analyses and copper content determination demonstrated Af _AO1 to belong to the multi-copper oxidase family. Biochemical characterization and 3D-modeling revealed a similarity to ascorbate oxidases, but also to laccases. Af _AO1 had a 10-fold higher affinity to ascorbic acid ( K |
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| معلومات مُعتمدة: | SC16-019 NÖ Forschungs- und Bildungsges.m.b.H.; 872161 Austrian Centre of Industrial Biotechnology; Open Access Publishing Fund Universität für Bodenkultur Wien |
| فهرسة مساهمة: | Keywords: ABTS; ascorbate oxidase; laccase; multi-copper oxidase |
| المشرفين على المادة: | 789U1901C5 (Copper) EC 1.10.3.2 (Laccase) EC 1.10.3.3 (Ascorbate Oxidase) |
| تواريخ الأحداث: | Date Created: 20200816 Date Completed: 20210217 Latest Revision: 20210217 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7460845 |
| DOI: | 10.3390/ijms21165754 |
| PMID: | 32796622 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms21165754 |