دورية أكاديمية
[Regulation of Gene Expression of Cancer/Testis Antigens in Colorectal Cancer Patients].
| العنوان: | [Regulation of Gene Expression of Cancer/Testis Antigens in Colorectal Cancer Patients]. |
|---|---|
| المؤلفون: | Kutilin DS; National Medical Research Centre for Oncology, Rostov-on-Don, 344037 Russia.; k.denees@yandex.ru. |
| المصدر: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2020 Jul-Aug; Vol. 54 (4), pp. 580-595. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | DNA Copy Number Variations* , Gene Expression Regulation, Neoplastic*, Antigens, Neoplasm/*genetics , Colorectal Neoplasms/*genetics , MicroRNAs/*genetics, DNA Methyltransferase 3A ; Gene Expression Regulation ; Humans ; Male |
| مستخلص: | The transcriptional activity of genes encoding cancer/testis antigens (CTA) and its regulation in colorectal cancer (CRC) is not well understood. The expression of CTA coding genes (CT genes) and possible mechanisms for its regulation, including expression and copy number of DNA methyltransferase genes, copy number of CT genes, microRNA expression, and LINE-1 methylation in CRC were analyzed in this study. The relative expression levels and copy number variation of 19 genes, MAGE-A1, -A2, -A3, -A4, -B1, -B2, GAGE-1, -3, -4, MAGEC1, BAGE, XAGE3, NY-ESO1, SSX2, SCP1, PRAME1, DNMT1, DNMT3A, and DNMT3B, were determined using real-time quantitative PCR. Quantitative methylation of LINE-1 CpG sites was evaluated by pyrosequencing, and multiple parallel sequencing was used to determine the level of microRNA expression. It was found that in colon tumor tissue a multidirectional destabilization of the transcriptional activity of DNMT3A and DNMT3B, associated with copy number variation and a change in expression of the CT genes BAGE, SSX2 and PRAME1, is observed. A strong positive correlation was found between copy number and expression of the BAGE, SSX2, and PRAME1 genes. As a result of multiple parallel sequencing, 6 differentially expressed microRNAs (hsa-miR-143-3p, hsa-miR-26a-5p, hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-21-5p, and hsa-let-7i-5p), targeting the CT genes GAGE1, SSX2, PRAME, SCP1, and the gene for DNA methyltransferase 3A (DNMT3A), were found. Data on the mechanisms of the transcriptional activity regulation of CT genes in malignant colon tumors are important for the development of CTA-dependent immunotherapeutic approaches for the treatment of this type of tumor. |
| فهرسة مساهمة: | Keywords: DNA methyltransferases; cancer/testis antigens; colorectal cancer; copy number; gene expression; methylation; microRNA; retrotransposons |
| المشرفين على المادة: | 0 (Antigens, Neoplasm) 0 (DNMT3A protein, human) 0 (MicroRNAs) EC 2.1.1.37 (DNA Methyltransferase 3A) |
| تواريخ الأحداث: | Date Created: 20200818 Date Completed: 20201214 Latest Revision: 20211204 |
| رمز التحديث: | 20221213 |
| DOI: | 10.31857/S0026898420040096 |
| PMID: | 32799221 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0026-8984 |
|---|---|
| DOI: | 10.31857/S0026898420040096 |