دورية أكاديمية
أعرض في EDS

Generation of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea.

التفاصيل البيبلوغرافية
العنوان: Generation of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea.
المؤلفون: Ndzeshang LB; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Fombad FF; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Njouendou AJ; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Chunda VC; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Gandjui NVT; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Akumtoh DN; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Chounna PWN; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Steven A; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom., Pionnier NP; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom., Layland LE; Institute for Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Bonn, Germany.; German Centre for Infection Research (DZIF), Partner Site, Bonn-Cologne, Bonn, Germany., Ritter M; Institute for Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Bonn, Germany., Hoerauf A; Institute for Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Bonn, Germany.; German Centre for Infection Research (DZIF), Partner Site, Bonn-Cologne, Bonn, Germany., Taylor MJ; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom., Turner JD; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom., Wanji S; Parasites and Vector Biology research unit (PAVBRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2020 Aug 17; Vol. 14 (8), pp. e0008415. Date of Electronic Publication: 2020 Aug 17 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Disease Vectors*, Diptera/*parasitology , Larva/*parasitology , Loa/*isolation & purification , Loiasis/*parasitology, Animals ; Disease Models, Animal ; Female ; Humans ; Larva/growth & development ; Life Cycle Stages ; Loa/cytology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microfilariae ; Survival Rate
مستخلص: Basic and translational research on loiasis, a filarial nematode infection of medical importance, is impeded by a lack of suitable Loa loa infection models and techniques of obtaining and culturing life cycle stages. We describe the development of a new method for routine production of infective third-stage larvae (L3) of L. loa from the natural intermediate arthropod vector host, Chrysops silacea, following experimental infection with purified microfilariae. At 14-days post-infection of C. silacea, the fly survival rate was 43%. Survival was significantly higher in flies injected with 50 mf (55.2%) than those that received 100 mf (31.0%). However, yield per surviving fly and total yield of L3 was markedly higher in the group of flies inoculated with 100 mf (3474 vs 2462 L3 produced). The abdominal segment hosted the highest percentage recovery of L3 (47.7%) followed by head (34.5%) and thorax (17.9%). L. loa larval survival was higher than 90% after 30 days of in vitro culture. The in vitro moulting success rate to the L4 larval stage was 59.1%. After experimental infection of RAG2-/-IL-2γc-/-mice, the average L. loa juvenile adult worm recovery rate was 10.5% at 62 dpi. More than 87% of the worms were recovered from the muscles and subcutaneous tissues. Worms recovered measured an average 24.3 mm and 11.4 mm in length for females (n = 5) and males (n = 5), respectively. In conclusion, L. loa mf injected into C. silacea intrathoracically develop into infective larvae that remain viable and infective comparable to L3 obtained through natural feeding on the human host. This technique further advances the development of a full laboratory life cycle of L. loa where mf derived from experimentally-infected animals may be utilized to passage life cycle generations via intrathoracic injections of wild-caught vector hosts.
Competing Interests: The authors have declared that no competing interests exist.
References: Parasit Vectors. 2018 Apr 4;11(1):223. (PMID: 29615094)
Am J Trop Med Hyg. 1975 Jul;24(4):610-5. (PMID: 1057379)
Ann Trop Med Parasitol. 2002 Dec;96(8):823-30. (PMID: 12625937)
Trans R Soc Trop Med Hyg. 2008 Oct;102(10):983-9. (PMID: 18466939)
Filaria J. 2006 May 10;5:6. (PMID: 16686951)
Trop Med Int Health. 2002 Apr;7(4):371-7. (PMID: 11952954)
Mem Inst Oswaldo Cruz. 1999 Sep-Oct;94(5):591-6. (PMID: 10464399)
Lancet. 1986 Jul 5;2(8497):32-3. (PMID: 2873327)
Nat Commun. 2019 Mar 29;10(1):1429. (PMID: 30926803)
PLoS Negl Trop Dis. 2015 Nov 10;9(11):e0004202. (PMID: 26555070)
Parasit Vectors. 2018 May 2;11(1):275. (PMID: 29716646)
Trends Parasitol. 2006 Jun;22(6):244-6. (PMID: 16632406)
PLoS Negl Trop Dis. 2011 Jun;5(6):e1210. (PMID: 21738809)
PLoS Negl Trop Dis. 2014 Sep 18;8(9):e3180. (PMID: 25232954)
Parasit Vectors. 2016 May 05;9:267. (PMID: 27151313)
Parasite. 1998 Mar;5(1):37-46. (PMID: 9754295)
Parasite. 2008 Jun;15(2):111-9. (PMID: 18642503)
Parasit Vectors. 2012 Feb 21;5:43. (PMID: 22348321)
Ann Trop Med Parasitol. 2001 Jul;95(5):495-507. (PMID: 11487371)
Trop Med Parasitol. 1989 Mar;40(1):57-64. (PMID: 2740729)
Ann Trop Med Parasitol. 1958 Mar;52(1):103-21. (PMID: 13521711)
Ann Soc Belg Med Trop. 1986;66(1):77-8. (PMID: 3459401)
Vet Parasitol. 1990 Jul;36(3-4):325-32. (PMID: 2399652)
Sci Transl Med. 2015 May 6;7(286):286re4. (PMID: 25947164)
J Med Entomol. 2006 Nov;43(6):1222-6. (PMID: 17162957)
تواريخ الأحداث: Date Created: 20200818 Date Completed: 20201005 Latest Revision: 20201005
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7470323
DOI: 10.1371/journal.pntd.0008415
PMID: 32804951
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0008415