دورية أكاديمية
Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time.
| العنوان: | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time. |
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| المؤلفون: | Barnes KG; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. kbarnes@broadinstitute.org.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA. kbarnes@broadinstitute.org.; MRC-University of Glasgow Centre for Virus Research, Glasgow, UK. kbarnes@broadinstitute.org., Lachenauer AE; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. annalach@stanford.edu.; Stanford University School of Medicine, Stanford, California, USA. annalach@stanford.edu., Nitido A; Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, 02139, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Siddiqui S; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Gross R; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Beitzel B; Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter Street, Fort Detrick, Maryland, 21702, USA., Siddle KJ; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Freije CA; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Dighero-Kemp B; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Mehta SB; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA., Carter A; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Uwanibe J; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Ajogbasile F; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Olumade T; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Odia I; Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Sandi JD; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone., Momoh M; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone., Metsky HC; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, Massachusetts, 02139, USA., Boehm CK; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Lin AE; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Kemball M; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Park DJ; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Branco L; Zalgen Labs, Germantown, Maryland, USA., Boisen M; Zalgen Labs, Germantown, Maryland, USA., Sullivan B; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA., Amare MF; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA., Tiamiyu AB; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation Medical Research International, Abuja, Nigeria., Parker ZF; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA., Iroezindu M; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation Medical Research International, Abuja, Nigeria., Grant DS; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone.; Ministry of Health and Sanitation, Freetown, Sierra Leone., Modjarrad K; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Myhrvold C; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Garry RF; Zalgen Labs, Germantown, Maryland, USA.; Tulane University School of Medicine, New Orleans, Los Angeles, 70112, USA., Palacios G; Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter Street, Fort Detrick, Maryland, 21702, USA., Hensley LE; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Schaffner SF; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Happi CT; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.; Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Colubri A; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Sabeti PC; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.; Howard Hughes Medical Institute, Chevy Chase, Maryland, USA. |
| المصدر: | Nature communications [Nat Commun] 2020 Aug 17; Vol. 11 (1), pp. 4131. Date of Electronic Publication: 2020 Aug 17. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Ebolavirus/*pathogenicity , Hemorrhagic Fever, Ebola/*diagnosis , Lassa Fever/*diagnosis , Lassa virus/*pathogenicity, Antibodies, Viral ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Ebolavirus/genetics ; Hemorrhagic Fever, Ebola/virology ; Lassa Fever/virology ; Lassa virus/genetics |
| مستخلص: | Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. We demonstrate on laboratory and clinical samples the sensitivity of these assays and the capacity of the SHERLOCK platform to handle virus-specific diagnostic challenges. We perform safety testing to demonstrate the efficacy of our HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extraction. We develop a user-friendly protocol and mobile application (HandLens) to report results, facilitating SHERLOCK's use in endemic regions. Finally, we successfully deploy our tests in Sierra Leone and Nigeria in response to recent outbreaks. |
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| معلومات مُعتمدة: | R01 AI114855 United States AI NIAID NIH HHS; United States HHMI Howard Hughes Medical Institute; R01 AI132223 United States AI NIAID NIH HHS; K01 TW010853 United States TW FIC NIH HHS; R01 AI132244 United States AI NIAID NIH HHS; U01 AI151812 United States AI NIAID NIH HHS; U54 HG007480 United States HG NHGRI NIH HHS; U19 AI110818 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20200819 Date Completed: 20200908 Latest Revision: 20210817 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7431545 |
| DOI: | 10.1038/s41467-020-17994-9 |
| PMID: | 32807807 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-020-17994-9 |