دورية أكاديمية
Ultradeformable Lipid Vesicles Localize Amphotericin B in the Dermis for the Treatment of Infectious Skin Diseases.
| العنوان: | Ultradeformable Lipid Vesicles Localize Amphotericin B in the Dermis for the Treatment of Infectious Skin Diseases. |
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| المؤلفون: | Fernández-García R, Statts L; Biomaterials, Bio-engineering and Nanomedicines (BioN) Laboratory, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Pharmaceutical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth, United Kingdom., de Jesus JA; Laboratory of Pathology of Infectious Diseases (LIM-50), Medical School, University of São Paulo, Avenida Dr. Arnaldo 455, 01246903 Cerqueira César, SP, Brazil., Dea-Ayuela MA; Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Carrer Santiago Ramón y Cajal s/n, 46113 Valencia, Spain., Bautista L; Biomaterials, Bio-engineering and Nanomedicines (BioN) Laboratory, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Pharmaceutical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth, United Kingdom., Simão R, Bolás-Fernández F, Ballesteros MP, Laurenti MD; Laboratory of Pathology of Infectious Diseases (LIM-50), Medical School, University of São Paulo, Avenida Dr. Arnaldo 455, 01246903 Cerqueira César, SP, Brazil., Passero LFD; São Paulo State University (UNESP), Institute of Biosciences, São Vicente Praça Infante Dom Henrique s/n, 11330-900 São Vicente, SP, Brazil.; São Paulo State University (UNESP), Institute for Advanced Studies of Ocean, São Vicente Av. João Francisco Bensdorp 1178, 11350-011 São Vicente, SP (Brazil)., Lalatsa A; Biomaterials, Bio-engineering and Nanomedicines (BioN) Laboratory, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Pharmaceutical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth, United Kingdom., Serrano DR |
| المصدر: | ACS infectious diseases [ACS Infect Dis] 2020 Oct 09; Vol. 6 (10), pp. 2647-2660. Date of Electronic Publication: 2020 Sep 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: ACS Publications Country of Publication: United States NLM ID: 101654580 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-8227 (Electronic) Linking ISSN: 23738227 NLM ISO Abbreviation: ACS Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ACS Publications, [2015]- |
| مواضيع طبية MeSH: | Antiprotozoal Agents* , Leishmaniasis, Cutaneous*/drug therapy, Amphotericin B/therapeutic use ; Animals ; Dermis ; Humans ; Lipids ; Mice |
| مستخلص: | Cutaneous fungal and parasitic diseases remain challenging to treat, as available therapies are unable to permeate the skin barrier. Thus, treatment options rely on systemic therapy, which fail to produce high local drug concentrations but can lead to significant systemic toxicity. Amphotericin B (AmB) is highly efficacious in the treatment of both fungal and parasitic diseases such as cutaneous leishmaniasis but is reserved for parenteral administration in patients with severe pathophysiology. Here, we have designed and optimized AmB-transfersomes [93.5% encapsulation efficiency, 150 nm size, and good colloidal stability (-35.02 mV)] that can remain physicochemically stable (>90% drug content) at room temperature and 4 °C over 6 months when lyophilized and stored under desiccated conditions. AmB-transfersomes possessed good permeability across mouse skin (4.91 ± 0.41 μg/cm 2 /h) and 10-fold higher permeability across synthetic Strat-M membranes. In vivo studies after a single topical application in mice showed permeability and accumulation within the dermis (>25 μg AmB/g skin 6 h postadministration), indicating the delivery of therapeutic amounts of AmB for mycoses and cutaneous leishmaniasis, while a single daily administration in Leishmania (Leishmania) amazonensis infected mice over 10 days, resulted in excellent efficacy (98% reduction in Leishmania parasites). Combining the application of AmB-transfersomes with metallic microneedles in vivo increased the levels in the SC and dermis but was unlikely to elicit transdermal levels. In conclusion, AmB-transfersomes are promising and stable topical nanomedicines that can be readily translated for parasitic and fungal infectious diseases. |
| فهرسة مساهمة: | Keywords: amphotericin B; fungal infections; leishmaniasis; tape stripping; transfersomes; ultradeformable lipid vesicles |
| المشرفين على المادة: | 0 (Antiprotozoal Agents) 0 (Lipids) 7XU7A7DROE (Amphotericin B) |
| تواريخ الأحداث: | Date Created: 20200819 Date Completed: 20210623 Latest Revision: 20210623 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acsinfecdis.0c00293 |
| PMID: | 32810398 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2373-8227 |
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| DOI: | 10.1021/acsinfecdis.0c00293 |