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Geometric principles underlying the proliferation of a model cell system.

التفاصيل البيبلوغرافية
العنوان: Geometric principles underlying the proliferation of a model cell system.
المؤلفون: Wu LJ; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK. l.j.wu@ncl.ac.uk., Lee S; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.; Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, SE5 9RX, UK., Park S; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.; Interdisciplinary Computing and Complex BioSystems research group, School of Computing, Newcastle University, Newcastle upon Tyne, NE4 5TG, UK., Eland LE; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.; Interdisciplinary Computing and Complex BioSystems research group, School of Computing, Newcastle University, Newcastle upon Tyne, NE4 5TG, UK., Wipat A; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.; Interdisciplinary Computing and Complex BioSystems research group, School of Computing, Newcastle University, Newcastle upon Tyne, NE4 5TG, UK., Holden S; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK., Errington J; Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK. jeff.errington@ncl.ac.uk.
المصدر: Nature communications [Nat Commun] 2020 Aug 18; Vol. 11 (1), pp. 4149. Date of Electronic Publication: 2020 Aug 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Bacillus subtilis/*growth & development , Cell Division/*physiology , Chromosome Segregation/*physiology , L Forms/*growth & development , Lab-On-A-Chip Devices/*microbiology, Bacillus subtilis/cytology ; Bacillus subtilis/physiology ; Cell Wall/physiology ; Chromosomes, Bacterial/metabolism ; Chromosomes, Bacterial/physiology ; L Forms/cytology ; L Forms/physiology ; Models, Biological
مستخلص: Many bacteria can form wall-deficient variants, or L-forms, that divide by a simple mechanism that does not require the FtsZ-based cell division machinery. Here, we use microfluidic systems to probe the growth, chromosome cycle and division mechanism of Bacillus subtilis L-forms. We find that forcing cells into a narrow linear configuration greatly improves the efficiency of cell growth and chromosome segregation. This reinforces the view that L-form division is driven by an excess accumulation of surface area over volume. Cell geometry also plays a dominant role in controlling the relative positions and movement of segregating chromosomes. Furthermore, the presence of the nucleoid appears to influence division both via a cell volume effect and by nucleoid occlusion, even in the absence of FtsZ. Our results emphasise the importance of geometric effects for a range of crucial cell functions, and are of relevance for efforts to develop artificial or minimal cell systems.
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 209500/Z/17/Z United Kingdom WT_ Wellcome Trust; 206670/Z/17/Z United Kingdom WT_ Wellcome Trust; 209500 United Kingdom WT_ Wellcome Trust
تواريخ الأحداث: Date Created: 20200820 Date Completed: 20200915 Latest Revision: 20230209
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7434903
DOI: 10.1038/s41467-020-17988-7
PMID: 32811832
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-020-17988-7