دورية أكاديمية
Comparison of a Novel Rapid Lateral Flow Assay to Enzyme Immunoassay Results for Early Diagnosis of Coccidioidomycosis.
| العنوان: | Comparison of a Novel Rapid Lateral Flow Assay to Enzyme Immunoassay Results for Early Diagnosis of Coccidioidomycosis. |
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| المؤلفون: | Donovan FM; The Valley Fever Center for Excellence, University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA.; The University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA., Ramadan FA; Department of Epidemiology and Biostatistics, University of Arizona College of Public Health, Tucson, Arizona, USA., Khan SA; Department of Epidemiology and Biostatistics, University of Arizona College of Public Health, Tucson, Arizona, USA., Bhaskara A; The University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA., Lainhart WD; The University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA.; Department of Pathology, University of Arizona College of Medicine-Tucson, Tucson Arizona, USA., Narang AT; University of Arizona College of Medicine-Phoenix, and Banner-University Medical Center Phoenix, Phoenix, Arizona, USA., Mosier JM; The University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA., Ellingson KD; Department of Epidemiology and Biostatistics, University of Arizona College of Public Health, Tucson, Arizona, USA., Bedrick EJ; Department of Epidemiology and Biostatistics, University of Arizona College of Public Health, Tucson, Arizona, USA., Saubolle MA; University of Arizona College of Medicine-Phoenix, and Banner-University Medical Center Phoenix, Phoenix, Arizona, USA., Galgiani JN; The Valley Fever Center for Excellence, University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA.; The University of Arizona College of Medicine-Tucson, Tucson, Arizona, USA. |
| المصدر: | Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [Clin Infect Dis] 2021 Nov 02; Vol. 73 (9), pp. e2746-e2753. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 9203213 Publication Model: Print Cited Medium: Internet ISSN: 1537-6591 (Electronic) Linking ISSN: 10584838 NLM ISO Abbreviation: Clin Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: Chicago, IL : The University of Chicago Press, c1992- |
| مواضيع طبية MeSH: | Coccidioidomycosis*/diagnosis, Early Diagnosis ; Humans ; Immunoassay ; Immunoenzyme Techniques ; Sensitivity and Specificity |
| مستخلص: | Background: Coccidioidomycosis (CM) is a common cause of community-acquired pneumonia where CM is endemic. Manifestations include self-limited pulmonary infection, chronic fibrocavitary pulmonary disease, and disseminated coccidioidomycosis. Most infections are identified by serological assays including enzyme-linked immunoassay (EIA), complement fixation, and immunodiffusion. These are time-consuming and take days to result, impeding early diagnosis. A new lateral flow assay (LFA; Sōna; IMMY, Norman, OK) improves time-to-result to 1 hour. Methods: We prospectively enrolled 392 patients with suspected CM, compared the LFA with standard EIA and included procalcitonin evaluation. Results: Compared with standard EIA, LFA demonstrates 31% sensitivity (95% confidence interval [CI], 20-44%) and 92% specificity (95% CI, 88-95%). Acute pulmonary disease (74%) was the most common clinical syndrome. Hospitalized patients constituted 75% of subjects, and compared with outpatients, they more frequently had ≥3 previous healthcare facility visits (P = .05), received antibacterials (P < .01), and had >3 antibacterial courses (P < .01). Procalcitonin (PCT) was <0.25 ng/mL in 52 (83%) EIA-positive patients, suggesting infection was not bacterial. Conclusions: When CM is a possible diagnosis, LFA identified nearly one-third of EIA-positive infections. Combined with PCT <0.25 ng/mL, LFA could reduce unnecessary antibacterial use by 77%. (© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 75D30118C02899 United States CC CDC HHS |
| فهرسة مساهمة: | Keywords: coccidioidomycosis; enzyme immunoassay (EIA); lateral flow assay (LFA); procalcitonin (PCT); valley fever |
| تواريخ الأحداث: | Date Created: 20200821 Date Completed: 20211110 Latest Revision: 20211110 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/cid/ciaa1205 |
| PMID: | 32818956 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-6591 |
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| DOI: | 10.1093/cid/ciaa1205 |