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Nitric oxide effects on Rhodnius prolixus's immune responses, gut microbiota and Trypanosoma cruzi development.

التفاصيل البيبلوغرافية
العنوان: Nitric oxide effects on Rhodnius prolixus's immune responses, gut microbiota and Trypanosoma cruzi development.
المؤلفون: Batista KKDS; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Vieira CS; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Florentino EB; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Caruso KFB; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Teixeira PTP; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Moraes CDS; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil., Genta FA; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil., de Azambuja P; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil; Programa de Pós-Graduação em Ciências e Biotecnologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., de Castro DP; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, RJ, Brazil; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. Electronic address: danicastro@ioc.fiocruz.br.
المصدر: Journal of insect physiology [J Insect Physiol] 2020 Oct; Vol. 126, pp. 104100. Date of Electronic Publication: 2020 Aug 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 2985080R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1611 (Electronic) Linking ISSN: 00221910 NLM ISO Abbreviation: J Insect Physiol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Elsevier
Original Publication: London ; New York : Pergamon Press
مواضيع طبية MeSH: Nitric Oxide*/metabolism , Nitric Oxide*/pharmacology , Rhodnius*/immunology , Rhodnius*/microbiology , Rhodnius*/parasitology, Gastrointestinal Microbiome/*drug effects , Immune System/*drug effects , Trypanosoma cruzi/*drug effects, Animals ; Arginine/antagonists & inhibitors ; Arginine/pharmacology ; Catalase/drug effects ; Catalase/metabolism ; Gastrointestinal Tract/drug effects ; Gastrointestinal Tract/microbiology ; Gene Expression/drug effects ; Genes, Insect ; Hemolymph/drug effects ; Hemolymph/immunology ; Hemolymph/metabolism ; Immunity, Humoral/drug effects ; Insect Vectors/immunology ; Insect Vectors/microbiology ; Insect Vectors/parasitology ; Monophenol Monooxygenase/drug effects ; Monophenol Monooxygenase/metabolism ; NG-Nitroarginine Methyl Ester/pharmacology ; Nitric Oxide Synthase/genetics ; Nitric Oxide Synthase/metabolism
مستخلص: The immune system of Rhodnius prolixus comprehends the synthesis of different effectors that modulate the intestinal microbiota population and the life cycle of the parasite Trypanosoma cruzi inside the vector midgut. One of these immune responses is the production of reactive nitrogen species (RNS) derived by the action of nitric oxide synthase (NOS). Therefore, we investigated the effects of L-arginine, the substrate for nitric oxide (NO) production and N ω -Nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of NOS, added in the insect blood meal. We analyzed the impact of these treatments on the immune responses and development of intestinal bacteria and parasites on R. prolixus nymphs. The L-arginine treatment in R. prolixus nymphs induced a higher NOS gene expression in the fat body and increased NO production, but reduced catalase and antimicrobial activities in the midgut. As expected, L-NAME treatment reduced NOS gene expression in the fat body. In addition, L-NAME treatment diminished catalase activity in the hemolymph and posterior midgut reduced phenoloxidase activity in the anterior midgut and increased the antimicrobial activity in the hemolymph. Both treatments caused a reduction in the cultivatable intestinal microbiota, especially in insects treated with L-NAME. However, T. cruzi development in the insect's digestive tract was suppressed after L-arginine treatment and the opposite was observed with L-NAME, which resulted in higher parasite counts. Therefore, we conclude that induction and inhibition of NOS and NO production are associated with other R. prolixus humoral immune responses, such as catalase, phenoloxidase, and antibacterial activities in different insect organs. These alterations reflect on intestinal microbiota and T. cruzi development.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antibacterial activity; Catalase; Nitric oxide synthase; Parasite; Phenoloxidase; Triatomine
المشرفين على المادة: 31C4KY9ESH (Nitric Oxide)
94ZLA3W45F (Arginine)
EC 1.11.1.6 (Catalase)
EC 1.14.13.39 (Nitric Oxide Synthase)
EC 1.14.18.1 (Monophenol Monooxygenase)
V55S2QJN2X (NG-Nitroarginine Methyl Ester)
تواريخ الأحداث: Date Created: 20200822 Date Completed: 20210119 Latest Revision: 20210119
رمز التحديث: 20221213
DOI: 10.1016/j.jinsphys.2020.104100
PMID: 32822690
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1611
DOI:10.1016/j.jinsphys.2020.104100