دورية أكاديمية
أعرض في EDS

A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi -Infected Human Cells.

التفاصيل البيبلوغرافية
العنوان: A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi -Infected Human Cells.
المؤلفون: Nova ICV; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil., Moreira LR; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, Brazil., Torres DJL; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, Brazil., Oliveira KKDS; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, Brazil., Patriota LLS; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil., Coelho LCBB; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil., Paiva PMG; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil., Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil., Lorena VMB; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, Brazil., Pontual EV; Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife 52171-900, Brazil.
المصدر: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2020 Aug 14; Vol. 9 (8). Date of Electronic Publication: 2020 Aug 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101637404 Publication Model: Electronic Cited Medium: Print ISSN: 2079-6382 (Print) Linking ISSN: 20796382 NLM ISO Abbreviation: Antibiotics (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Trypanosoma cruzi causes the lethal Chagas disease, which is endemic in Latin America. Flowers of Moringa oleifera (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to T. cruzi trypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by T. cruzi -infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LC 50 of 43.5 µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index > 11.48 was determined. When T. cruzi -infected PBMCs were treated with MoFTI (43.5 or 87.0 µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity to T. cruzi and the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease.
References: Front Microbiol. 2016 Apr 27;7:611. (PMID: 27199940)
Vaccines (Basel). 2019 Oct 12;7(4):. (PMID: 31614804)
Biomed Pharmacother. 2019 Mar;111:224-235. (PMID: 30584985)
Mol Biochem Parasitol. 1989 Feb;33(1):33-41. (PMID: 2651912)
Med Res Rev. 2018 Jul;38(4):1295-1331. (PMID: 29149530)
Nat Rev Immunol. 2018 Dec;18(12):773-789. (PMID: 30254251)
J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682)
Infect Dis Clin North Am. 2019 Mar;33(1):119-134. (PMID: 30712757)
J Diet Suppl. 2018 Nov 2;15(6):939-950. (PMID: 29345972)
J Immunol. 2012 Jan 15;188(2):649-60. (PMID: 22156594)
Front Chem. 2019 Nov 25;7:798. (PMID: 31824926)
Vet Parasitol. 2013 Dec 6;198(3-4):371-81. (PMID: 24129068)
Trials. 2020 Apr 15;21(1):328. (PMID: 32293523)
Int J Antimicrob Agents. 2018 Aug;52(2):185-194. (PMID: 29635008)
Acta Trop. 2016 Apr;156:1-16. (PMID: 26747009)
Front Plant Sci. 2015 Aug 25;6:655. (PMID: 26379683)
Front Immunol. 2016 Jan 18;6:659. (PMID: 26834737)
Curr Top Med Chem. 2002 Nov;2(11):1261-71. (PMID: 12171584)
J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
Mediators Inflamm. 2014;2014:912965. (PMID: 25104883)
Acta Trop. 2016 Dec;164:117-124. (PMID: 27619190)
Curr Pharm Des. 2002;8(4):269-85. (PMID: 11860366)
Molecules. 2020 Jun 17;25(12):. (PMID: 32560454)
Nat Prod Res. 2018 Dec;32(24):2940-2944. (PMID: 29047320)
Int J Mol Sci. 2020 Jun 16;21(12):. (PMID: 32560043)
J Ethnopharmacol. 2006 Apr 21;105(1-2):306-11. (PMID: 16386862)
Parasite Immunol. 2014 Aug;36(8):377-87. (PMID: 24611805)
Parasitol Res. 2014 Feb;113(2):727-33. (PMID: 24271154)
Plant Sci. 2020 Mar;292:110398. (PMID: 32005400)
Arthritis Res Ther. 2006;8 Suppl 2:S2. (PMID: 16899106)
معلومات مُعتمدة: 408789/2016-6 Conselho Nacional de Desenvolvimento Científico e Tecnológico; Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; APQ-0108-2.08/14 Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco; APQ-0661-2.08/15 Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
فهرسة مساهمة: Keywords: Moringa oleifera; cytokines; cytotoxicity; immunomodulatory agent; protease inhibitor; trypanocidal agent
تواريخ الأحداث: Date Created: 20200823 Latest Revision: 20200928
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7460158
DOI: 10.3390/antibiotics9080515
PMID: 32823803
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6382
DOI:10.3390/antibiotics9080515