دورية أكاديمية
Cellular Expression and Functional Roles of All 26 Neurotransmitter GPCRs in the C. elegans Egg-Laying Circuit.
| العنوان: | Cellular Expression and Functional Roles of All 26 Neurotransmitter GPCRs in the C. elegans Egg-Laying Circuit. |
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| المؤلفون: | Fernandez RW; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Wei K; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Wang EY; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Mikalauskaite D; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Olson A; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Pepper J; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Christie N; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Kim S; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510., Weissenborn S; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, 01307, Germany., Sarov M; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, 01307, Germany., Koelle MR; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510 michael.koelle@yale.edu. |
| المصدر: | The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2020 Sep 23; Vol. 40 (39), pp. 7475-7488. Date of Electronic Publication: 2020 Aug 26. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : Society for Neuroscience Original Publication: [Baltimore, Md.] : The Society, c1981- |
| مواضيع طبية MeSH: | Oviposition*, Caenorhabditis elegans Proteins/*metabolism , Neurons/*cytology , Neurotransmitter Agents/*metabolism , Receptors, G-Protein-Coupled/*metabolism, Animals ; Caenorhabditis elegans ; Caenorhabditis elegans Proteins/genetics ; Epithelial Cells/metabolism ; Epithelial Cells/physiology ; Neural Pathways/cytology ; Neural Pathways/metabolism ; Neural Pathways/physiology ; Neurons/metabolism ; Neurons/physiology ; Receptors, G-Protein-Coupled/genetics |
| مستخلص: | Maps of the synapses made and neurotransmitters released by all neurons in model systems, such as Caenorhabditis elegans have left still unresolved how neural circuits integrate and respond to neurotransmitter signals. Using the egg-laying circuit of C. elegans as a model, we mapped which cells express each of the 26 neurotransmitter GPCRs of this organism and also genetically analyzed the functions of all 26 GPCRs. We found that individual neurons express many distinct receptors, epithelial cells often express neurotransmitter receptors, and receptors are often positioned to receive extrasynaptic signals. Receptor knockouts reveal few egg-laying defects under standard laboratory conditions, suggesting that the receptors function redundantly or regulate egg-laying only in specific conditions; however, increasing receptor signaling through overexpression more efficiently reveals receptor functions. This map of neurotransmitter GPCR expression and function in the egg-laying circuit provides a model for understanding GPCR signaling in other neural circuits. SIGNIFICANCE STATEMENT Neurotransmitters signal through GPCRs to modulate activity of neurons, and changes in such signaling can underlie conditions such as depression and Parkinson's disease. To determine how neurotransmitter GPCRs together help regulate function of a neural circuit, we analyzed the simple egg-laying circuit in the model organism C. elegans We identified all the cells that express every neurotransmitter GPCR and genetically analyzed how each GPCR affects the behavior the circuit produces. We found that many neurotransmitter GPCRs are expressed in each neuron, that neurons also appear to use these receptors to communicate with other cell types, and that GPCRs appear to often act redundantly or only under specific conditions to regulate circuit function. (Copyright © 2020 the authors.) |
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| معلومات مُعتمدة: | T32 GM007223 United States GM NIGMS NIH HHS; P40 OD010440 United States OD NIH HHS; F31 NS115315 United States NS NINDS NIH HHS; R01 NS086932 United States NS NINDS NIH HHS; R01 NS036918 United States NS NINDS NIH HHS; T32 GM007499 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: C. elegans; GPCR; egg-laying circuit; metabotropic receptor; neural circuit |
| المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (Neurotransmitter Agents) 0 (Receptors, G-Protein-Coupled) |
| تواريخ الأحداث: | Date Created: 20200828 Date Completed: 20210115 Latest Revision: 20210324 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7511189 |
| DOI: | 10.1523/JNEUROSCI.1357-20.2020 |
| PMID: | 32847964 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-2401 |
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| DOI: | 10.1523/JNEUROSCI.1357-20.2020 |