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Isobutanol production freed from biological limits using synthetic biochemistry.

التفاصيل البيبلوغرافية
العنوان: Isobutanol production freed from biological limits using synthetic biochemistry.
المؤلفون: Sherkhanov S; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Korman TP; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA.; Invizyne Technologies, Inc., Monrovia, CA, USA., Chan S; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Faham S; Vertex Pharmaceuticals, Boston, MA, USA., Liu H; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Sawaya MR; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Hsu WT; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Vikram E; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Cheng T; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA., Bowie JU; Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California, Los Angeles, CA, USA. bowie@mbi.ucla.edu.
المصدر: Nature communications [Nat Commun] 2020 Aug 27; Vol. 11 (1), pp. 4292. Date of Electronic Publication: 2020 Aug 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Biochemistry/*methods , Butanols/*metabolism , Enzymes/*metabolism, Acetolactate Synthase/chemistry ; Acetolactate Synthase/metabolism ; Adenosine Triphosphate ; Alcohol Oxidoreductases/genetics ; Alcohol Oxidoreductases/metabolism ; Biochemistry/instrumentation ; Bioreactors ; Cell-Free System ; Directed Molecular Evolution ; Enzymes/chemistry ; Enzymes/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Glucose/metabolism ; Temperature ; Thermodynamics
مستخلص: Cost competitive conversion of biomass-derived sugars into biofuel will require high yields, high volumetric productivities and high titers. Suitable production parameters are hard to achieve in cell-based systems because of the need to maintain life processes. As a result, next-generation biofuel production in engineered microbes has yet to match the stringent cost targets set by petroleum fuels. Removing the constraints imposed by having to maintain cell viability might facilitate improved production metrics. Here, we report a cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity of 4 g L -1 h -1 , a titer of 275 g L -1 and 95% yield over the course of nearly 5 days. These production metrics exceed even the highly developed ethanol fermentation process. Our results suggest that moving beyond cells has the potential to expand what is possible for bio-based chemical production.
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المشرفين على المادة: 0 (Butanols)
0 (Enzymes)
0 (Escherichia coli Proteins)
56F9Z98TEM (isobutyl alcohol)
8L70Q75FXE (Adenosine Triphosphate)
EC 1.1.- (Alcohol Oxidoreductases)
EC 1.1.- (YahK protein, E coli)
EC 2.2.1.6 (Acetolactate Synthase)
IY9XDZ35W2 (Glucose)
تواريخ الأحداث: Date Created: 20200829 Date Completed: 20200924 Latest Revision: 20210827
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC7453195
DOI: 10.1038/s41467-020-18124-1
PMID: 32855421
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-020-18124-1