دورية أكاديمية
أعرض في EDS

Molecular Characterization of Hovenia Dulcis-Associated Virus 1 (HDaV1) and 2 (HDaV2): New Tentative Species within the Order Picornavirales .

التفاصيل البيبلوغرافية
العنوان: Molecular Characterization of Hovenia Dulcis-Associated Virus 1 (HDaV1) and 2 (HDaV2): New Tentative Species within the Order Picornavirales .
المؤلفون: Nery FMB; Departamento de Fitopatologia, Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília DF 70910-900, Brazil., Melo FL; Departamento de Fitopatologia, Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília DF 70910-900, Brazil., Boiteux LS; Embrapa Hortaliças, Brasília DF 70275-970, Brazil., Ribeiro SG; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Resende RO; Departamento de Biologia Celular, Campus Universitário Darcy Ribeiro, Universidade de Brasília (UnB), Brasília DF 70910-900, Brazil., Orílio AF; Departamento de Biologia Celular, Campus Universitário Darcy Ribeiro, Universidade de Brasília (UnB), Brasília DF 70910-900, Brazil., Batista JG; Departamento de Fitopatologia, Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília DF 70910-900, Brazil., Lima MF; Embrapa Hortaliças, Brasília DF 70275-970, Brazil., Pereira-Carvalho RC; Departamento de Fitopatologia, Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília DF 70910-900, Brazil.
المصدر: Viruses [Viruses] 2020 Aug 27; Vol. 12 (9). Date of Electronic Publication: 2020 Aug 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Dicistroviridae/*genetics , Picornaviridae/*genetics, Brazil ; Dicistroviridae/classification ; Dicistroviridae/isolation & purification ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Phylogeny ; Picornaviridae/classification ; Picornaviridae/isolation & purification ; Plant Diseases/virology ; Rhamnaceae/virology ; Viral Proteins/genetics
مستخلص: In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis ( Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis -associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis -associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales . HDaV2 was assigned as a novel species in the Dicistroviridae family. The 5' ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
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فهرسة مساهمة: Keywords: HDaV1; HDaV2; Hovenia dulcis; Picornavirales; metagenomics; virome
المشرفين على المادة: 0 (Viral Proteins)
تواريخ الأحداث: Date Created: 20200902 Date Completed: 20210308 Latest Revision: 20210308
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7552035
DOI: 10.3390/v12090950
PMID: 32867192
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v12090950