دورية أكاديمية
[Establishment of DNA fingerprints for Chrysosplenium using SRAP Markers].
| العنوان: | [Establishment of DNA fingerprints for Chrysosplenium using SRAP Markers]. |
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| المؤلفون: | Huang W; Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences, South-Central University for Nationalities Wuhan 430074, China., Lan DQ; Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences, South-Central University for Nationalities Wuhan 430074, China., Qin R; Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences, South-Central University for Nationalities Wuhan 430074, China., Liu H; Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences, South-Central University for Nationalities Wuhan 430074, China., Li G; Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences, South-Central University for Nationalities Wuhan 430074, China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2020 Aug; Vol. 45 (15), pp. 3659-3665. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | DNA Fingerprinting* , Genetic Variation*, Cluster Analysis ; Genetic Markers ; Phylogeny ; Polymorphism, Genetic |
| مستخلص: | As a traditional Chinese medicinal material, Chrysosplenium is urgently needed for genetic resource investigation and protection research due to the decrease of its wild resources in recent years. After investigating the wild resources, we conducted genetic polymorphism and clustering studies of 24 species(a total of 36 samples) of Chrysosplenium using SRAP technique. The results showed that a total of 374 polymorphic bands were obtained using 18 pairs of SRAP primers to amplify these samples, on average of 20.7 bands for each primer pair. We used the biological software to analyze the population's genetic parameter and got the N_a value as 2.000 0, N_(e )value as 1.408 4, the average Nei's index as 0.263 5, and the average Shannon information index as 0.419 1. UPGMA cluster analysis showed that all the samples can be divided into three major groups at the genetic similarity coefficient of 0.70: there are 18 species(24 samples) gathered for the Ⅰ groups, 3 species or variation(7 samples) for Ⅱ groups, and 3 species(5 samples) for Ⅲ groups. The differences of these Chrysosplenium species at the molecular level are consistent with that of their geographical and ecological distribution. At the same time, we used SRAP technology to construct 36 DNA digital fingerprints of Chrysosplenium and obtained the unique molecular identification band type of each material. These results will provide effective methods and reliable basis for the identification, protection and genetic diversity analysis of the germplasm resources of Chrysosplenium, and lay a foundation for the further development and utilization of them. |
| فهرسة مساهمة: | Keywords: Chrysosplenium; DNA fingerprints; SRAP; germplasm |
| المشرفين على المادة: | 0 (Genetic Markers) |
| تواريخ الأحداث: | Date Created: 20200907 Date Completed: 20200917 Latest Revision: 20200917 |
| رمز التحديث: | 20231215 |
| DOI: | 10.19540/j.cnki.cjcmm.20200527.105 |
| PMID: | 32893555 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20200527.105 |