دورية أكاديمية
Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR).
| العنوان: | Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR). |
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| المؤلفون: | Kofman A; Epidemic Intelligence Service, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA.; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Linderman S; Emory Vaccine Center, Emory University, Atlanta, Georgia, USA., Su K; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA., Purpura LJ; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Ervin E; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Brown S; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Morales-Betoulle M; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Graziano J; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Cannon DL; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Klena JD; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Desamu-Thorpe R; Office of Public Health Preparedness and Response, Center for Preparedness and Response, CDC, Atlanta, Georgia, USA., Fankhauser J; ELWA Hospital, Samaritan's Purse, Monrovia, Liberia.; Ventura County Medical Center, Ventura, California, USA., Orone R; ELWA Hospital, Samaritan's Purse, Monrovia, Liberia., Moses S; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Glaybo U; ELWA Hospital, Samaritan's Purse, Monrovia, Liberia., Massaquoi M; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Nyenswah T; National Public Health Institute of Liberia, Monrovia, Liberia., Nichol ST; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Kollie J; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Kiawu A; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Freeman E; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Giah G; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Tony H; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Faikai M; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Jawara M; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Kamara K; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Kamara S; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Flowers B; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Mohammed K; Ministry of Health Liberia, Men's Health Screening Program, Monrovia, Liberia., Chiriboga D; University of Massachusetts Medical School, Worcester, Massachusetts, USA., Williams DE; Center for Global Health, CDC, Atlanta, Georgia, USA., Hinrichs SH; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA., Ahmed R; Emory Vaccine Center, Emory University, Atlanta, Georgia, USA., Vonhm B; National Public Health Institute of Liberia, Monrovia, Liberia., Rollin PE; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA., Choi MJ; Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia, USA. |
| المصدر: | Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [Clin Infect Dis] 2021 Dec 06; Vol. 73 (11), pp. e3641-e3646. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 9203213 Publication Model: Print Cited Medium: Internet ISSN: 1537-6591 (Electronic) Linking ISSN: 10584838 NLM ISO Abbreviation: Clin Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: Chicago, IL : The University of Chicago Press, c1992- |
| مواضيع طبية MeSH: | Ebolavirus*/genetics , Hemorrhagic Fever, Ebola*, Antibodies, Viral ; Humans ; Leukocytes, Mononuclear ; Liberia/epidemiology ; Male ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse Transcription ; Semen ; Survivors |
| مستخلص: | Introduction: Ebola virus (EBOV), species Zaire ebolavirus, may persist in the semen of male survivors of Ebola virus disease (EVD). We conducted a study of male survivors of the 2014-2016 EVD outbreak in Liberia and evaluated their immune responses to EBOV. We report here findings from the serologic testing of blood for EBOV-specific antibodies, molecular testing for EBOV in blood and semen, and serologic testing of peripheral blood mononuclear cells (PBMCs) in a subset of study participants. Methods: We tested for EBOV RNA in blood by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and for anti-EBOV-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) for 126 study participants. We performed PBMC analysis on a subgroup of 26 IgG-negative participants. Results: All 126 participants tested negative for EBOV RNA in blood by qRT-PCR. The blood of 26 participants tested negative for EBOV-specific IgG antibodies by ELISA. PBMCs were collected from 23/26 EBOV IgG-negative participants. Of these, 1/23 participants had PBMCs that produced anti-EBOV-specific IgG antibodies upon stimulation with EBOV-specific glycoprotein (GP) and nucleoprotein (NP) antigens. Conclusions: The blood of EVD survivors, collected when they did not have symptoms meeting the case definition for acute or relapsed EVD, is unlikely to pose a risk for EBOV transmission. We identified 1 IgM/IgG negative participant who had PBMCs that produced anti-EBOV-specific antibodies upon stimulation. Immunogenicity following acute EBOV infection may exist along a spectrum, and absence of antibody response should not be exclusionary in determining an individual's status as a survivor of EVD. (Published by Oxford University Press for the Infectious Diseases Society of America 2020.) |
| معلومات مُعتمدة: | CDC Foundation; United States CC CDC HHS |
| فهرسة مساهمة: | Keywords: Ebola survivors; immunology; semen persistence |
| المشرفين على المادة: | 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20200907 Date Completed: 20220314 Latest Revision: 20220314 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/cid/ciaa1331 |
| PMID: | 32894277 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-6591 |
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| DOI: | 10.1093/cid/ciaa1331 |