دورية أكاديمية
أعرض في EDS

Enhancing mucosal immunity by transient microbiota depletion.

التفاصيل البيبلوغرافية
العنوان: Enhancing mucosal immunity by transient microbiota depletion.
المؤلفون: Becattini S; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA. simone.becattini@gmail.com., Littmann ER; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA., Seok R; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Amoretti L; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Fontana E; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Wright R; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Gjonbalaj M; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Leiner IM; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA., Plitas G; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Ludwig Center at Memorial Sloan Kettering Cancer Center, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Breast Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Hohl TM; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Pamer EG; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA.
المصدر: Nature communications [Nat Commun] 2020 Sep 08; Vol. 11 (1), pp. 4475. Date of Electronic Publication: 2020 Sep 08.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Immunity, Mucosal*/drug effects, Gastrointestinal Microbiome/*immunology, Administration, Oral ; Animals ; Antigens/administration & dosage ; CD8-Positive T-Lymphocytes/immunology ; Chemotaxis/immunology ; Female ; Gastrointestinal Microbiome/drug effects ; Host Microbial Interactions/immunology ; Immunologic Memory ; Listeria monocytogenes/growth & development ; Listeria monocytogenes/immunology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Ovalbumin/administration & dosage ; Streptomycin/administration & dosage
مستخلص: Tissue resident memory CD8 +  T cells (Trm) are poised for immediate reactivation at sites of pathogen entry and provide optimal protection of mucosal surfaces. The intestinal tract represents a portal of entry for many infectious agents; however, to date specific strategies to enhance Trm responses at this site are lacking. Here, we present TMDI (Transient Microbiota Depletion-boosted Immunization), an approach that leverages antibiotic treatment to temporarily restrain microbiota-mediated colonization resistance, and favor intestinal expansion to high densities of an orally-delivered Listeria monocytogenes strain carrying an antigen of choice. By augmenting the local chemotactic gradient as well as the antigenic load, this procedure generates a highly expanded pool of functional, antigen-specific intestinal Trm, ultimately enhancing protection against infectious re-challenge in mice. We propose that TMDI is a useful model to dissect the requirements for optimal Trm responses in the intestine, and also a potential platform to devise novel mucosal vaccination approaches.
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معلومات مُعتمدة: P30 CA008748 United States CA NCI NIH HHS; R01 AI042135 United States AI NIAID NIH HHS; United States HHMI Howard Hughes Medical Institute
المشرفين على المادة: 0 (Antigens)
9006-59-1 (Ovalbumin)
Y45QSO73OB (Streptomycin)
تواريخ الأحداث: Date Created: 20200909 Date Completed: 20200921 Latest Revision: 20240428
رمز التحديث: 20240428
مُعرف محوري في PubMed: PMC7479140
DOI: 10.1038/s41467-020-18248-4
PMID: 32901029
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-020-18248-4