دورية أكاديمية
Enhancing mucosal immunity by transient microbiota depletion.
العنوان: | Enhancing mucosal immunity by transient microbiota depletion. |
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المؤلفون: | Becattini S; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA. simone.becattini@gmail.com., Littmann ER; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA., Seok R; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Amoretti L; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Fontana E; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Wright R; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Gjonbalaj M; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Leiner IM; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA., Plitas G; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Ludwig Center at Memorial Sloan Kettering Cancer Center, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Breast Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Hohl TM; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA., Pamer EG; Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Lucille Castori Center for Microbes Inflammation and Cancer, Molecular Microbiology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.; Duchossois Family Institute, University of Chicago, Chicago, IL, 60606, USA. |
المصدر: | Nature communications [Nat Commun] 2020 Sep 08; Vol. 11 (1), pp. 4475. Date of Electronic Publication: 2020 Sep 08. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
مواضيع طبية MeSH: | Immunity, Mucosal*/drug effects, Gastrointestinal Microbiome/*immunology, Administration, Oral ; Animals ; Antigens/administration & dosage ; CD8-Positive T-Lymphocytes/immunology ; Chemotaxis/immunology ; Female ; Gastrointestinal Microbiome/drug effects ; Host Microbial Interactions/immunology ; Immunologic Memory ; Listeria monocytogenes/growth & development ; Listeria monocytogenes/immunology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Ovalbumin/administration & dosage ; Streptomycin/administration & dosage |
مستخلص: | Tissue resident memory CD8 + T cells (Trm) are poised for immediate reactivation at sites of pathogen entry and provide optimal protection of mucosal surfaces. The intestinal tract represents a portal of entry for many infectious agents; however, to date specific strategies to enhance Trm responses at this site are lacking. Here, we present TMDI (Transient Microbiota Depletion-boosted Immunization), an approach that leverages antibiotic treatment to temporarily restrain microbiota-mediated colonization resistance, and favor intestinal expansion to high densities of an orally-delivered Listeria monocytogenes strain carrying an antigen of choice. By augmenting the local chemotactic gradient as well as the antigenic load, this procedure generates a highly expanded pool of functional, antigen-specific intestinal Trm, ultimately enhancing protection against infectious re-challenge in mice. We propose that TMDI is a useful model to dissect the requirements for optimal Trm responses in the intestine, and also a potential platform to devise novel mucosal vaccination approaches. |
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معلومات مُعتمدة: | P30 CA008748 United States CA NCI NIH HHS; R01 AI042135 United States AI NIAID NIH HHS; United States HHMI Howard Hughes Medical Institute |
المشرفين على المادة: | 0 (Antigens) 9006-59-1 (Ovalbumin) Y45QSO73OB (Streptomycin) |
تواريخ الأحداث: | Date Created: 20200909 Date Completed: 20200921 Latest Revision: 20240428 |
رمز التحديث: | 20240428 |
مُعرف محوري في PubMed: | PMC7479140 |
DOI: | 10.1038/s41467-020-18248-4 |
PMID: | 32901029 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2041-1723 |
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DOI: | 10.1038/s41467-020-18248-4 |