دورية أكاديمية
Zinc-protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers.
| العنوان: | Zinc-protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers. |
|---|---|
| المؤلفون: | Santos NR; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Bandeira MJ; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Bah HAF; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Rodrigues JLG; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Cardoso MS; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Rocha AR; Graduate Program in Food Science, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil., Menezes-Filho JA; Graduate Program in Pharmacy, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil.; Graduate Program in Food Science, College of Pharmacy, Federal University of Bahia. Salvador, Bahia, Brazil. |
| المصدر: | Biomedical chromatography : BMC [Biomed Chromatogr] 2021 Feb; Vol. 35 (2), pp. e4983. Date of Electronic Publication: 2020 Oct 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: England NLM ID: 8610241 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-0801 (Electronic) Linking ISSN: 02693879 NLM ISO Abbreviation: Biomed Chromatogr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1990- : Chichester : Wiley Original Publication: London : Heyden & Son, c1986-1990 |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid/*methods , Lead/*blood , Occupational Exposure/*analysis , Protoporphyrins/*blood, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers/blood ; Child ; Female ; Hemoglobins/analysis ; Humans ; Linear Models ; Male ; Middle Aged ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Fluorescence ; Young Adult |
| مستخلص: | Lead (Pb) exposure compromises heme synthesis by inhibiting ferrochelatase, forming zinc-protoporphyrin (ZnPP). This study aims to validate a method for the determination of ZnPP by HPLC with fluorescence detection and apply this method to evaluate the extent of Pb exposure of artisanal pottery workers. Extraction procedures were tested using both nonacid and acid liquid-liquid extraction. The former presented a better chromatogram and recovery results. The validated method yielded a good resolution of ZnPP and its free form peaks with acceptable precision and accuracy. Total run time was 15 min and ZnPP peak retention time was 5.6 min. We applied this method to evaluate 39 potters (90% male), mean age 40 years (9-80). The medians (ranges) of blood lead, ZnPP and hemoglobin were 16.0 μg/dl (2.2-71.5), 12.6 μg/dl (4.6-279.8) and 15.1 g/dl (11.0-17.8), respectively. Significant differences were observed for blood lead according to gender, age range (>40 years), direct handling of lead oxide and years of occupation. Significantly higher levels of ZnPP were observed in male potters involved with lead glazing activity. The validated method was shown to be simple with one-step nonacid extraction, good sensitivity, reproducibility and accuracy. Our data shows that these laborers are dangerously exposed to Pb, reflecting the effect on the heme synthesis. (© 2020 John Wiley & Sons, Ltd.) |
| References: | ANVISA (Agência Nacional de Vigilância Sanitária). (2016). Reagentes. Retrieved from http://portal.anvisa.gov.br/documents/10181/2954708/Cap%C3%ADtulos%20do%20Volume%201%20-%2014%20Reagentes.pdf/c1fec423-78c1-477c-91d9-6b95fd2401e2. ATSDR (Agency for Toxic Substances and Disease Registry). (2019a). Lead toxicity. Atlanta, GA. Retrieved from: Environmental Health and Medicine Education. https://www.atsdr.cdc.gov/csem/csem.asp?csem=34%po=6. ATSDR (Agency for Toxic Substances and Disease Registry). (2019b). Toxicological profile for lead. Atlanta, GA: US Department of Health and Human Services. Retrieved from https://www.atsdr.cdc.gov/toxprofiles/tp13.pdf. Bianchi, P. D., Barp, J., Thomé, F. S., & Belló-Klein, A. (2009). Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais. Jornal Brasileiro de Nefrologia, 31, 175-182. Brasil. Ministério do Trabalho, Portaria n. 24 de 29.12.1994. (1994). Norma Regulamentadora n. 7. Programa de Controle Médico de Saúde Ocupacional. Diário Oficial da União de 30.12.1994, Brasília. Carvalho, F. M., Silvany Neto, A. M., Peres, M. F. T., Gonçalves, H. R. G., Amorim, G. C., Silva, C. J. B. Jr., & Mascarenhas, T. (1996). Intoxicação pelo chumbo: zinco protoporfirina no sangue de crianças de Santo Amaro da Purificação e de Salvador, BA. Jornal de Pediatria, 72, 295-298. https://doi.org/10.2223/jped.629. Center for Disease Control and Prevention. (2015). Adult blood lead epidemiology and surveillance. US Department of Health and Human Services, National Institute for Occupational Safety and Health Division of Surveillance, Atlanta, GA. Retrieved from https://www.cdc.gov/niosh/topics/ables/description.html. Costa, R. G., Bah, H. A. F., Bandeira, M. J., Oliveira, S. S. P., & Menezes-Filho, J. A. (2017). Lead and cadmium in mangrove root crab (Goniopsis cruentata), in natura and at food processing stages. Food Additives & Contaminants: Part B, 10, 216-221. Counter, S. A., Buchanan, L. H., & Ortega, F. (2008). Zinc protoporphyrin levels, blood lead levels and neurocognitive deficits in Andrean children with chronic lead exposure. Clinical Biochemistry, 41, 41-47. https://doi.org/10.1016/j.clinbiochem.2007.10.002. Cypriani, M. G. S. C., Alves, A. N. L., Bechara, E. J. H., & Assunção, N. A. (2014). Bioanalytical studies of porphyric disorders using HPLC with fluorescence detection. Journal of the Brazilian Chemical Society, 10, 1918-1926. Dally, S. (1988). Chumbo e saúde: alguns dados recentes. Documentos Para O Trabalho Do médico, 33, 9-15. D'Souza, H. S., Menezes, G., Dsouza, S. A., & Vankatesh, T. (2015). Evaluation of symptoms and characteristic features of lead poisoning and their assistance in clinical decision making. International Journal of Clinical Therapeutics and Diagnosis, 3, 97-99. EMA (European Medicines Agency). (2011). Guideline on bioanalytical method validation. Canary Wharf, UK: Committee for Medicinal Products for Human Use. Fischbein, A., Thornton, J. C., Lilis, R., Valciukas, J. A., Bernstein, J., & Selikoff, I. J. (1980). Zinc protoporphyrin, blood lead and clinical symptoms in two occupational groups with low-level exposure to lead. American Journal of Industrial Medicine, 1, 391-399. https://doi.org/10.1002/ajim.4700010316. Flora, S. J. S. (2014). Metals. Biomarkers in. Toxicology, 485-519. FONART (Fomento de las Artesanías, Gobierno de México). (2017). Ollas y jarritos de barro sin plomo. Retrieved from https://www.gob.mx/salud/articulos/ollas-y-jarritos-de-barro-sin-plomo. Froom, P., Kristal-Boneh, E., Benbassat, J., Ashkanazi, R., & Ribak, J. (1998). Predictive value of determinations of zinc protoporphyrin for increased blood lead concentrations. Clinical Chemistry, 44, 1283-1288. Ho, J., Guthrie, R., & Tieckelmann, H. (1987). Quantitative determination of porphyrins, their precursors and zinc protoporphyrin in whole blood and dried blood by high-performance liquid chromatography with fluorimetric detection. Journal of Chromatogrophy, 417, 269-276. Karacic, V., Prpic-Majic, D., & Telisman, S. (1980). The relationship between zinc protoporphyrin and free erythrocyte protoporphyrin in lead exposed individuals. International Archives of Occupational and Environmental Health, 47, 165-177. https://doi.org/10.1007/BF00716375. Kasuba, V., Rozgaj, R., Milic, M., Zeljezic, D., Nopjar, N., Pizent, A., … Jazbec, A. (2012). Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay. International Archives of Occupational and Environmental Health, 85, 807-818. https://doi.org/10.1007/s00420-011-0726-4. Klaassen, C. D. (1991). Metais Pesados e seus Antagonistas. In A. Gilman (Ed.), Goodman et al. as bases farmacológicas da terapêutica (8th ed.). Rio de Janeiro: Guanabara Koogan. Labbé, R. F., Vreman, H. J., & Stevenson, D. K. (1999). Zinc protoporphyrin: A metabolite with a mission. Clinical Chemistry, 45, 2060-2072. Lamola, A. A., Joselow, M., & Yamane, T. (1975). Zinc protoporphyrin: A simple, sensitive, fluorometric screening test for lead poisoning. Clinical Chemistry, 21, 93-97. Martin, C. J., Werntz, C. L., & Ducatman, A. M. (2004). The interpretation of zinc protoporphyrin changes in lead intoxication: A case report and review of literature. Occupational Medicine, 54, 587-591. https://doi.org/10.1093/occmed/kqh123. Menezes-Filho, J. A., Carvalho, W. A., & Spínola, A. G. (2003). Avaliação da exposição ocupacional ao chumbo em uma metalúrgica: um estudo transversal. Revista Brasileira de Saúde Ocupacional, São Paulo, 29, 63-72. Menezes-Filho, J. A., Viana, G. F. S., & Paes, C. R. (2012). Determinants of lead exposure in children on the outskirts of Salvador, Brazil. Environmental Monitoring Assessment, 184, 2593-2603. https://doi.org/10.1007/s10661-011-2137-0. Molin, F. D., Paoliello, M. M. B., & De Capitani, E. M. (2006). A zincoprotoporfirina como indicador biológico na exposição ao chumbo: uma revisão. Revista Brasileira de Toxicologia, 19, 71-80. Molina-Ballesteros, G., Zúñiga-Charles, M. A., Cárdenas Ortega, A., Solís-Cámara, R., J, P., & Solís-Cámara, V. P. (1983). Lead concentrations in the blood of children from pottery-making families exposed to lead salts in a Mexican village. Bulletin of the Pan American Health Organization (PAHO), 17, 35-41. Ortiz-Ortiz, E., García-Nieto, E., Juaárez-Santacruz, L., Gómez-Camarillo, M. A., García-Gallegos, E., & Limón-Huitrón, P. (2017). Lead exposure: Pottery impact in Tlaxcala, Mexico. Revista Internacional de Contaminacion Ambiental, 33, 57-64. Pachathundikandi, S. K., & Varghese, E. T. (2006). Blood zinc protoporphyrin, serum total protein, and total cholesterol levels in automobile workshop workers in relation to lead toxicity: Our experience. Indian Journal of Clinical Biochemistry, 21, 114-117. https://doi.org/10.1007/BF02912924. Quitério, S. L., Moreira, F. R., Silva, C. R. S., Arbilla, G., Araújo, U. C., & Mattos, R. C. O. C. (2006). Avaliação da poluição ambiental causada por particulado de chumbo emitido por uma reformadora de baterias na cidade do Rio de Janeiro, Brasil. Caderno de Saúde Pública, 22, 1817-1823. Sadão, M. (2002). Intoxicação por chumbo. Revista de Oxidologia, 11, 37-42. Sakai, T. (2000). Biomarkers of lead exposure. Industrial Health, 38, 127-142. https://doi.org/10.2486/indhealth.38.127. Souza, A. M., & Tavares, C. F. F. (2009). Chumbo e anemia. Ribeirão Preto: Revista de Medicina da USP, 42, 337-340. World Health Organization. (2001). Iron deficiency anaemia: Assessment, prevention and control. A guide for programme managers. Department of Nutrition for Health and Development. Retrieved from https://www.who.int/nutrition/publications/micronutrients/anaemia_iron_deficiency/WHO_NHD_01.3/en/. World Health Organization. (2019). Lead poisoning and health. Retrieved from https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health. Young-Man, R., Kangyoon, K., & Hyunwook, K. (2000). Zinc protoporphyrin IX concentrations between normal adults and the lead exposed workers measured by HPLC, spectrofluorometer and hematofluorometer. Industrial Health, 38, 372-379. |
| معلومات مُعتمدة: | JCB0029/2015 Fundação de Apoio a Pesquisa do Estado da Bahia, Brazil (FAPESB); JCB 0029/2015 Fundação de Apoio a Pesquisa do Estado da Bahia, Brazil (FAPESB) |
| فهرسة مساهمة: | Keywords: HPLC; fluorescence; lead; occupational exposure; zinc-protoporphyrin |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Hemoglobins) 0 (Protoporphyrins) 15442-64-5 (zinc protoporphyrin) 2P299V784P (Lead) |
| تواريخ الأحداث: | Date Created: 20200910 Date Completed: 20210524 Latest Revision: 20210524 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/bmc.4983 |
| PMID: | 32909293 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-0801 |
|---|---|
| DOI: | 10.1002/bmc.4983 |