دورية أكاديمية
Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control.
| العنوان: | Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control. |
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| المؤلفون: | Stock R; Molecular Biology and Neurobiology, NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany., Vogel S; Molecular Biology and Neurobiology, NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany., Mau-Holzmann UA; Institute of Medical Genetics and Applied Genomics, University of Tübingen, Calwerstr. 7, 72076 Tübingen, Germany., Kriebel M; Molecular Biology and Neurobiology, NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany., Wüst R; Department of Psychiatry, University of Tübingen, Osianderstr. 24, 72076 Tübingen, Germany., Fallgatter AJ; Department of Psychiatry, University of Tübingen, Osianderstr. 24, 72076 Tübingen, Germany., Volkmer H; Molecular Biology and Neurobiology, NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany. Electronic address: volkmer@nmi.de. |
| المصدر: | Stem cell research [Stem Cell Res] 2020 Oct; Vol. 48, pp. 101961. Date of Electronic Publication: 2020 Aug 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kidlington, Oxford : Elsevier |
| مواضيع طبية MeSH: | Induced Pluripotent Stem Cells* , Schizophrenia*/genetics, Case-Control Studies ; Cell Differentiation ; Cell Line ; Cellular Reprogramming ; Fibroblasts ; Humans |
| مستخلص: | Fibroblasts were isolated from skin biopsies of four patients diagnosed with schizophrenia and from one healthy control. Patient fibroblasts were transfected with five episomal, non-integrative reprogramming vectors to generate human induced pluripotent stem cells (iPSC). Reprogrammed iPSC showed consistent expression of several pluripotency markers, loss of expression of exogenous reprogramming vectors and ability to differentiate into all three germ layers. Additionally, iPSC maintained their normal karyotype during reprogramming. These generated cell lines can be used to study early neurodevelopmental and neuroinflammatory processes in schizophrenia in a patient-derived in vitro model. (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.) |
| تواريخ الأحداث: | Date Created: 20200910 Date Completed: 20210528 Latest Revision: 20210528 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.scr.2020.101961 |
| PMID: | 32911325 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1876-7753 |
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| DOI: | 10.1016/j.scr.2020.101961 |