دورية أكاديمية
Azadirachtin interferes with basal immunity and microbial homeostasis in the Rhodnius prolixus midgut.
| العنوان: | Azadirachtin interferes with basal immunity and microbial homeostasis in the Rhodnius prolixus midgut. |
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| المؤلفون: | Vieira CS; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil., Figueiredo MB; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil., Moraes CDS; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil., Pereira SB; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil., Dyson P; School of Medicine, Swansea University, Swansea, UK., Mello CB; Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niteroi, RJ, Brazil; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil; Programa de Pós-Graduação Em Ciências e Biotecnologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Castro DP; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil., Azambuja P; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil; Programa de Pós-Graduação Em Ciências e Biotecnologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil. Electronic address: Patriciaazambuja@id.uff.br. |
| المصدر: | Developmental and comparative immunology [Dev Comp Immunol] 2021 Jan; Vol. 114, pp. 103864. Date of Electronic Publication: 2020 Sep 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004. |
| مواضيع طبية MeSH: | Chagas Disease/*immunology , Ecdysone/*metabolism , Insect Proteins/*metabolism , Insect Vectors/*physiology , Insecticides/*administration & dosage , Intestinal Mucosa/*immunology , Limonins/*administration & dosage , Rhodnius/*physiology , Trypanosoma cruzi/*physiology , Trypanosoma rangeli/*physiology, Animals ; Drosophila Proteins/metabolism ; Gastrointestinal Microbiome ; Homeostasis ; Immunity, Humoral ; Immunity, Innate ; Molting ; NF-kappa B/metabolism ; Serratia marcescens ; Signal Transduction |
| مستخلص: | Rhodnius prolixus is an insect vector of two flagellate parasites, Trypanosoma rangeli and Trypanosoma cruzi, the latter being the causative agent of Chagas disease in Latin America. The R. prolixus neuroendocrine system regulates the synthesis of the steroid hormone ecdysone, which is essential for not only development and molting but also insect immunity. Knowledge for how this modulates R. prolixus midgut immune responses is essential for understanding interactions between the vector, its parasites and symbiotic microbes. In the present work, we evaluated the effects of ecdysone inhibition on R. prolixus humoral immunity and homeostasis with its microbiota, using the triterpenoid natural product, azadirachtin. Our results demonstrated that azadirachtin promoted a fast and lasting inhibitory effect on expression of both RpRelish, a nuclear factor kappa B transcription factor (NF-kB) component of the IMD pathway, and several antimicrobial peptide (AMP) genes. On the other hand, RpDorsal, encoding the equivalent NF-kB transcription factor in the Toll pathway, and the defC AMP gene were upregulated later in azadirachtin treated insects. The treatment also impacted on proliferation of Serratia marcescens, an abundant commensal bacterium. The simultaneous administration of ecdysone and azadirachtin in R. prolixus blood meals counteracted the azadirachtin effects on insect molting and also on expression of RpRelish and AMPs genes. These results support the direct involvement of ecdysone in regulation of the IMD pathway in the Rhodnius prolixus gut. (Copyright © 2020. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Antimicrobial peptides; Ecdysone; Immune signaling pathways; Microbiota; Rhodnius prolixus; Serratia marcescens |
| المشرفين على المادة: | 0 (Drosophila Proteins) 0 (Insect Proteins) 0 (Insecticides) 0 (Limonins) 0 (NF-kappa B) 0 (imd protein, Drosophila) 3604-87-3 (Ecdysone) O4U1SAF85H (azadirachtin) |
| تواريخ الأحداث: | Date Created: 20200912 Date Completed: 20220209 Latest Revision: 20220209 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.dci.2020.103864 |
| PMID: | 32918931 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0089 |
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| DOI: | 10.1016/j.dci.2020.103864 |