دورية أكاديمية
Knockdown of fatty acid binding protein 4 exacerbates Bacillus Calmette-Guerin infection-induced RAW264.7 cell apoptosis via the endoplasmic reticulum stress pathway.
| العنوان: | Knockdown of fatty acid binding protein 4 exacerbates Bacillus Calmette-Guerin infection-induced RAW264.7 cell apoptosis via the endoplasmic reticulum stress pathway. |
|---|---|
| المؤلفون: | Yu J; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China., Ma C; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China., Xu Y; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China., Han L; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China., Wu X; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China. Electronic address: wuxiaol@nxu.edu.cn., Wang Y; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China. Electronic address: wyj@nxu.edu.cn., Deng G; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in western China, NingXia University, NingXia, Yinchuan 750021, PR China; School of Life Science, NingXia University, NingXia, Yinchuan 750021, PR China. Electronic address: dgc@nxu.edu.cn. |
| المصدر: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2020 Nov; Vol. 85, pp. 104552. Date of Electronic Publication: 2020 Sep 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Apoptosis*/genetics , Endoplasmic Reticulum Stress*/genetics , Signal Transduction*, Fatty Acid-Binding Proteins/*genetics , Mycobacterium bovis/*physiology, Animals ; Fatty Acid-Binding Proteins/metabolism ; Fatty Acids/metabolism ; Gene Knockdown Techniques ; Host-Pathogen Interactions/genetics ; Mice ; Oxidation-Reduction ; RAW 264.7 Cells ; Reactive Oxygen Species/metabolism ; Tuberculosis/genetics ; Tuberculosis/metabolism ; Tuberculosis/microbiology |
| مستخلص: | Mycobacterial infection can induce alveolar macrophage apoptosis, which plays a vital role in the pathogenesis of tuberculosis. Accumulating evidence has demonstrated that fatty acid oxidation is involved in apoptosis during various pathological processes, including bacterial infection. However, whether fatty acid oxidation regulates mycobacterial infection-induced macrophage apoptosis remains unclear. Hence, the present study aimed to investigate the role of fatty acid binding protein 4 (FABP4) which is a carrier protein for fatty acids, in regulating apoptosis in RAW264.7 cells infected with Bacillus Calmette-Guerin (BCG). In our study, the impact of BCG infection on apoptosis and fatty acid oxidation in RAW264.7 cells was examined. Notably, we found that FABP4 was overexpressed during this process. Furthermore, small interfering RNAs targeting FABP4 were used to investigate the role of FABP4 in regulating apoptosis and fatty acid oxidation in BCG-infected RAW264.7 cells. The results indicated that mycobacterial infection promoted apoptosis and enhanced fatty acid oxidation in RAW264.7 cells. Moreover, FABP4 knockdown exacerbated BCG-induced apoptosis and upregulated the expression of p-PERK, p-eIF2α and chop, which are endoplasmic reticulum (ER) stress markers. In addition, FABP4 knockdown promoted fatty acid oxidation and ROS production, which result in the activation of ER stress. Our data suggested that FABP4 knockdown exacerbated BCG-induced apoptosis in RAW264.7 cells via the ER stress pathway. (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Endoplasmic reticulum stress; FABP4; Fatty acid oxidation |
| المشرفين على المادة: | 0 (Fabp4 protein, mouse) 0 (Fatty Acid-Binding Proteins) 0 (Fatty Acids) 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20200913 Date Completed: 20211104 Latest Revision: 20211104 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.meegid.2020.104552 |
| PMID: | 32920196 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1567-7257 |
|---|---|
| DOI: | 10.1016/j.meegid.2020.104552 |