دورية أكاديمية
Th17/Treg imbalance in COPD development: suppressors of cytokine signaling and signal transducers and activators of transcription proteins.
| العنوان: | Th17/Treg imbalance in COPD development: suppressors of cytokine signaling and signal transducers and activators of transcription proteins. |
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| المؤلفون: | Silva LEF; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Lourenço JD; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Silva KR; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Santana FPR; Laboratory of Studies in Pulmonary Inflammation, Department of Bioscience, Federal University of Sao Paulo, Diadema, SP, Brazil., Kohler JB; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Moreira AR; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Velosa APP; Laboratory of Extracelular Matrix, Department of Clinical Medicine, School of Medicine of University of Sao Paulo, Sao Paulo, SP, Brazil., Prado CM; Laboratory of Studies in Pulmonary Inflammation, Department of Bioscience, Federal University of Sao Paulo, Santos, SP, Brazil., Vieira RP; Post-Graduation Program in Bioengineering, Universidade Brasil, Sao Paulo, SP, Brazil., Aun MV; Host & Defense Unit, Faculdade Israelita de Ciências da Saúde Albert Einstein, Sao Paulo, SP, Brazil., Tibério IFLC; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Ito JT; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil., Lopes FDTQS; Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil. fernandadtqsl@gmail.com.; Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455 - room 1220, Sao Paulo, SP, 01246-903, Brazil. fernandadtqsl@gmail.com. |
| المصدر: | Scientific reports [Sci Rep] 2020 Sep 17; Vol. 10 (1), pp. 15287. Date of Electronic Publication: 2020 Sep 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Cytokines/*immunology , Pulmonary Disease, Chronic Obstructive/*immunology , Signal Transduction/*immunology , T-Lymphocytes, Regulatory/*immunology , Th17 Cells/*immunology, Animals ; Disease Progression ; Down-Regulation/immunology ; Inflammation/immunology ; Male ; Mice ; Mice, Inbred C57BL ; STAT3 Transcription Factor/immunology ; Suppressor of Cytokine Signaling 1 Protein/immunology ; Suppressor of Cytokine Signaling 3 Protein/immunology |
| مستخلص: | Th17/Treg imbalance contributes to chronic obstructive pulmonary disease (COPD) development and progression. However, intracellular signaling by suppressor of cytokine signaling (SOCS) 1 and SOCS3 and the proteins signal transducer and activator of transcription (STAT) 3 and STAT5 that orchestrate these imbalances are currently poorly understood. Thus, these proteins were investigated in C57BL/6 mice after exposure to cigarette smoke (CS) for 3 and 6 months. The expression of interleukin was measured by ELISA and the density of positive cells in peribronchovascular areas was quantified by immunohistochemistry. We showed that exposure to CS in the 3rd month first induced decreases in the numbers of STAT5+ and pSTAT5+ cells and the expression levels of TGF-β and IL-10. The increases in the numbers of STAT3+ and pSTAT3+ cells and IL-17 expression occurred later (6th month). These findings corroborate the increases in the number of SOCS1+ cells in both the 3rd and 6th months, with concomitant decreases in SOCS3+ cells at the same time points. Our results demonstrated that beginning with the initiation of COPD development, there was a downregulation of the anti-inflammatory response mediated by SOCS and STAT proteins. These results highlight the importance of intracellular signaling in Th17/Treg imbalance and the identification of possible targets for future therapeutic approaches. |
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| المشرفين على المادة: | 0 (Cytokines) 0 (STAT3 Transcription Factor) 0 (Suppressor of Cytokine Signaling 1 Protein) 0 (Suppressor of Cytokine Signaling 3 Protein) |
| تواريخ الأحداث: | Date Created: 20200918 Date Completed: 20201204 Latest Revision: 20210917 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7499180 |
| DOI: | 10.1038/s41598-020-72305-y |
| PMID: | 32943702 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-020-72305-y |