دورية أكاديمية
Structural characterization, antifungal and cytotoxic profiles of quaternized heteropolysaccharide from Anadenanthera colubrina.
| العنوان: | Structural characterization, antifungal and cytotoxic profiles of quaternized heteropolysaccharide from Anadenanthera colubrina. |
|---|---|
| المؤلفون: | Ribeiro FOS; Research Center on Biodiversity and Biotechnology, BIOTEC, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil; Center for Research in Applied Morphology and Immunology, NuPMIA, University of Brasilia, Brasilia, Brazil., de Araújo GS; Research Center on Biodiversity and Biotechnology, BIOTEC, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil., Mendes MGA; Group of Advanced Studies in Medical Mycology, GEAMICOL, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil., Daboit TC; Group of Advanced Studies in Medical Mycology, GEAMICOL, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil., Brito LM; Department of Physiology and Pharmacology, Federal University of Ceará, UFC, Fortaleza, CE, Brazil., Pessoa C; Department of Physiology and Pharmacology, Federal University of Ceará, UFC, Fortaleza, CE, Brazil., de Lima LRM; Department of Organic and Inorganic Chemistry, Federal University of Ceará, UFC, Fortaleza, CE, Brazil., de Paula RCM; Department of Organic and Inorganic Chemistry, Federal University of Ceará, UFC, Fortaleza, CE, Brazil., Bastos RS; Research Group in Medicinal Chemistry and Biotechnology, QUIMEBIO, Federal University of Maranhão, UFMA, São Bernardo, MA, Brazil., Rocha JA; Research Group in Medicinal Chemistry and Biotechnology, QUIMEBIO, Federal University of Maranhão, UFMA, São Bernardo, MA, Brazil., de Brito Sa E; Research Center on Biodiversity and Biotechnology, BIOTEC, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil., de Oliveira TC; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, PE, Brazil., de Jesus Oliveira AC; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, PE, Brazil., Sobrinho JLS; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, PE, Brazil., de Souza de Almeida Leite JR; Center for Research in Applied Morphology and Immunology, NuPMIA, University of Brasilia, Brasilia, Brazil., de Araújo AR; Research Center on Biodiversity and Biotechnology, BIOTEC, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil., da Silva DA; Research Center on Biodiversity and Biotechnology, BIOTEC, Federal University of the Parnaíba Delta, UFDPar, Parnaíba, PI, Brazil. Electronic address: durcileneas@gmail.com. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 15; Vol. 165 (Pt A), pp. 279-290. Date of Electronic Publication: 2020 Sep 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Antifungal Agents*/chemistry , Antifungal Agents*/pharmacology , Cytotoxins*/chemistry , Cytotoxins*/pharmacology , Enzyme Inhibitors*/chemistry , Enzyme Inhibitors*/pharmacology , Fungal Proteins*/antagonists & inhibitors , Fungal Proteins*/chemistry , Molecular Docking Simulation* , Polysaccharides*/chemistry , Polysaccharides*/pharmacology, Fabaceae/*chemistry , Fungi/*enzymology, Animals ; HEK293 Cells ; Humans ; Ligases/antagonists & inhibitors ; Ligases/chemistry ; Mice |
| مستخلص: | In the present work, we investigated the minimal inhibitory concentration (MIC) against fungal strains (Fonsecaea pedrosoi, Microsporum canis, Candida albicans, Cryptococcus neoformans), and cytotoxicity to normal cell lines for modified red angico gum (AG) with eterifying agent N-chloride (3-chloro-2-hydroxypropyl) trimethylammonium (CHPTAC). Quaternized ammonium groups were linked to AG backbone using N-(3-chloro-2-hydroxypropyl) trimethylammonium chloride. The chemical features of the quaternized gum derivatives (QAG) were analyzed by: FTIR, elemental analysis, Zeta potential and gel permeation chromatography. The angico quaternizated gum presented a degree of substitution (DS) of 0.22 and Zeta potential of +36.43. For the antifungal test, it was observed that unmodified gum did not inhibit fungal growth. While, QAG inhibited the growth of most fungi used in this study. By AFM technique QAG interacted with the fungal surface, altering wall roughness significantly. The probable affinity of fragments of the QAG structure for the fungal enzyme 5I33 (Adenylosuccinate synthetase) has been shown by molecular docking. Low cytotoxicity was observed for polymers (unmodified gum and QAG). The results demonstrate that the quaternized polymer of AG presented in this study is a quite promising biomaterial for biotechnological applications. Competing Interests: Declaration of competing interest There are no conflicts of interest to declare. (Copyright © 2018. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Antimicrobial; Biocompatibility; Chemically modified polymer |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Cytotoxins) 0 (Enzyme Inhibitors) 0 (Fungal Proteins) 0 (Polysaccharides) EC 6.- (Ligases) |
| تواريخ الأحداث: | Date Created: 20200921 Date Completed: 20210408 Latest Revision: 20210408 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijbiomac.2020.09.087 |
| PMID: | 32956746 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
|---|---|
| DOI: | 10.1016/j.ijbiomac.2020.09.087 |