دورية أكاديمية
A cold water, ultrasonically activated stream efficiently removes proteins and prion-associated amyloid from surgical stainless steel.
| العنوان: | A cold water, ultrasonically activated stream efficiently removes proteins and prion-associated amyloid from surgical stainless steel. |
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| المؤلفون: | Secker TJ; Environmental Healthcare Unit, School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK. Electronic address: tjs1x07@soton.ac.uk., Leighton TG; Institute of Sound and Vibration Research, Faculty of Engineering and Environment, University of Southampton, Southampton, UK; Sloan Water Technology Ltd, Chilworth, Southampton, UK., Offin DG; Chemistry, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK., Birkin PR; Chemistry, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK., Hervé RC; Environmental Healthcare Unit, School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK., Keevil CW; Environmental Healthcare Unit, School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK. |
| المصدر: | The Journal of hospital infection [J Hosp Infect] 2020 Dec; Vol. 106 (4), pp. 649-656. Date of Electronic Publication: 2020 Sep 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: W.B. Saunders For The Hospital Infection Society Country of Publication: England NLM ID: 8007166 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2939 (Electronic) Linking ISSN: 01956701 NLM ISO Abbreviation: J Hosp Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : W.B. Saunders For The Hospital Infection Society Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Equipment Contamination* , Stainless Steel* , Ultrasonics*, Decontamination/*methods , Prions/*isolation & purification, Surgical Instruments ; Water |
| مستخلص: | Background: Sterile service department decontamination procedures for surgical instruments struggle to demonstrate efficient removal of the hardiest infectious contaminants, such as prion proteins. A recently designed novel system, which uses a low pressure ultrasonically activated, cold water stream, has previously demonstrated efficient hard surface cleaning of several biological contaminants. Aim: To test the efficacy of an ultrasonically activated stream for the removal of tissue proteins, including prion-associated amyloid, from surgical stainless steel surfaces. Methods: Test surfaces were contaminated with 22L, ME7 or 263K prion-infected brain homogenates. The surfaces were treated with the ultrasonically activated water stream for contact times of 5 and 10 s. Residual proteinaceous and amyloid contamination were quantified using sensitive microscopic analysis, and immunoblotting was used to characterize the eluted prion residues before and after treatment with the ultrasonically activated stream. Findings: Efficient removal of the different prion strains from the surgical stainless steel surfaces was observed, and reduced levels of protease-susceptible and -resistant prion protein was detected in recovered supernatant. Conclusion: This study demonstrated that an ultrasonically activated stream has the potential to be a cost-effective solution to improve current decontamination practices and has the potential to reduce hospital-acquired infections. (Copyright © 2020 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Creutzfeldt–Jakob disease; Decontamination; Prion; Protein; Surgical; Ultrasonic cleaning |
| المشرفين على المادة: | 0 (Prions) 059QF0KO0R (Water) 12597-68-1 (Stainless Steel) |
| تواريخ الأحداث: | Date Created: 20200921 Date Completed: 20210713 Latest Revision: 20210713 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7501313 |
| DOI: | 10.1016/j.jhin.2020.09.021 |
| PMID: | 32956784 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2939 |
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| DOI: | 10.1016/j.jhin.2020.09.021 |