دورية أكاديمية
Immunophenotypic characteristics of juvenile myelomonocytic leukaemia and their relation with the molecular subgroups of the disease.
| العنوان: | Immunophenotypic characteristics of juvenile myelomonocytic leukaemia and their relation with the molecular subgroups of the disease. |
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| المؤلفون: | Frisanco Oliveira A; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; Brazilian Co-operative Study Group for Paediatric Myelodysplastic Syndrome (GCB-SMD-PED) - Morphology and Flow Cytometry Committee, Barretos, São Paulo, Brazil., Tansini A; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; GCB-SMD-PED - Flow cytometry Committee, Barretos, São Paulo, Brazil., Toledo TR; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; GCB-SMD-PED - Flow cytometry Committee, Barretos, São Paulo, Brazil., Balceiro R; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; Brazilian Co-operative Study Group for Paediatric Myelodysplastic Syndrome (GCB-SMD-PED) - Morphology and Flow Cytometry Committee, Barretos, São Paulo, Brazil., Onofre Vidal D; GCB-SMD-PED - Molecular Biology and Genetic Committee, Barretos, São Paulo, Brazil., de Martino Lee ML; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; GCB-SMD-PED - Morphology and Myeloproliferative Diseases Committee, Barretos, São Paulo, Brazil., Lorand-Metze I; Brazilian Co-operative Study Group for Paediatric Myelodysplastic Syndrome (GCB-SMD-PED) - Morphology and Flow Cytometry Committee, Barretos, São Paulo, Brazil.; GCB-SMD-PED - Chairman, Barretos, São Paulo, Brazil., Lopes LF; Barretos Children's Cancer Hospital, Barretos, São Paulo, Brazil.; GCB-SMD-PED - Chairman, Barretos, São Paulo, Brazil. |
| المصدر: | British journal of haematology [Br J Haematol] 2021 Jan; Vol. 192 (1), pp. 129-136. Date of Electronic Publication: 2020 Sep 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 0372544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2141 (Electronic) Linking ISSN: 00071048 NLM ISO Abbreviation: Br J Haematol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Wiley-Blackwell Original Publication: Oxford : Blackwell Scientific Publications |
| مواضيع طبية MeSH: | Immunophenotyping*, Leukemia, Myelomonocytic, Juvenile/*diagnosis, Antigens, CD/analysis ; Antigens, CD/genetics ; Antigens, CD/immunology ; Bone Marrow/immunology ; Bone Marrow/pathology ; Child, Preschool ; Female ; Gene Expression Regulation, Neoplastic ; Granulocytes/immunology ; Granulocytes/pathology ; Humans ; Infant ; Leukemia, Myelomonocytic, Juvenile/genetics ; Leukemia, Myelomonocytic, Juvenile/immunology ; Male |
| مستخلص: | The diagnosis of juvenile myelomonocytic leukaemia (JMML) is based on clinical, laboratory and molecular features but immunophenotyping [multiparametric flow cytometry (MFC)] has not been used routinely. In the present study, we describe the flow cytometric features at diagnosis with special attention to the distribution of monocytic subsets and the relation between MFC and molecular subgroups. MFC was performed with an eight-colour platform based on Euroflow. We studied 33 JMML cases. CD34 + /CD117 + /CD13 + cells >2% was found in 25 cases, and 51·5% presented an aberrant expression of CD7. A decrease of CD34 + /CD19 + /CD10 + cells was seen in eight cases and in four they were absent. The granulocytic population had a decreased side scatter in 29 cases. Bone marrow monocytic precursors were increased in 28 patients, with a decrease in classical monocytes (median 80·7%) and increase in CD16 + (intermediate and non-classical). A more pronounced increase in myeloid CD34 + cells was seen in patients with Neurofibromatosis type 1 (NF1) and tyrosine-protein phosphatase non-receptor type 11 (PTPN11), with aberrant CD7 expression in four of six and 10/12 patients respectively. Thus, JMML shows an immunophenotypic profile similar to myelodysplastic syndromes, and a different monocyte subset distribution when compared with chronic MML. MFC proved to be an important diagnostic tool that can help in differential diagnosis with other clonal diseases with monocytosis. (© 2020 British Society for Haematology and John Wiley & Sons Ltd.) |
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| فهرسة مساهمة: | Keywords: CMML; flow cytometry; juvenile myelomonocytic leukaemia; molecular subtypes; myelodysplastic syndrome |
| المشرفين على المادة: | 0 (Antigens, CD) |
| تواريخ الأحداث: | Date Created: 20200923 Date Completed: 20210426 Latest Revision: 20210426 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/bjh.17098 |
| PMID: | 32966606 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2141 |
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| DOI: | 10.1111/bjh.17098 |