دورية أكاديمية
An investigation of transient intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli.
| العنوان: | An investigation of transient intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli. |
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| المؤلفون: | Grant PL, Basford JM, John RA |
| المصدر: | The Biochemical journal [Biochem J] 1987 Feb 01; Vol. 241 (3), pp. 699-704. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 2984726R Publication Model: Print Cited Medium: Print ISSN: 0264-6021 (Print) Linking ISSN: 02646021 NLM ISO Abbreviation: Biochem J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London, UK : Published by Portland Press on behalf of the Biochemical Society |
| مواضيع طبية MeSH: | Glutamate Decarboxylase/*metabolism , Glutamates/*metabolism, Escherichia coli/enzymology ; Fluorescence Polarization ; Kinetics ; Spectrophotometry |
| مستخلص: | Several intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli were detected by stopped-flow spectrophotometry and by rapid-scanning spectrophotometry after conventional mixing. Structures were assigned to intermediates on the basis of kinetic and spectral evidence. In the early stages of the reaction an intermediate with the properties expected of a geminal diamine accumulated significantly. Changes consistent with the conversion of this species into the external aldimine were also observed. The course of product formation was determined and linked with spectral changes taking place in the bound coenzyme. The effect of the minor decarboxylation-dependent transamination that accompanies the major reaction was analysed. |
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| المشرفين على المادة: | 0 (Glutamates) 470-51-9 (2-methylglutamic acid) EC 4.1.1.15 (Glutamate Decarboxylase) |
| تواريخ الأحداث: | Date Created: 19870201 Date Completed: 19870716 Latest Revision: 20190501 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC1147620 |
| DOI: | 10.1042/bj2410699 |
| PMID: | 3297037 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0264-6021 |
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| DOI: | 10.1042/bj2410699 |