دورية أكاديمية
Laboratory methods supporting measles surveillance in Queensland, Australia, 2010-2017.
| العنوان: | Laboratory methods supporting measles surveillance in Queensland, Australia, 2010-2017. |
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| المؤلفون: | McMahon JL; Child Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia.; Public Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia., Northill JA; Public Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia., Finger M; Public Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia., Lyon M; Public Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia., Lambert SB; Child Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia., Mackay IM; Child Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia.; Public Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia. |
| المصدر: | Access microbiology [Access Microbiol] 2020 Jan 27; Vol. 2 (3), pp. acmi000093. Date of Electronic Publication: 2020 Jan 27 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 101746927 Publication Model: eCollection Cited Medium: Internet ISSN: 2516-8290 (Electronic) Linking ISSN: 25168290 NLM ISO Abbreviation: Access Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Microbiology Society, [2019]- |
| مستخلص: | Purpose: Australia was officially recognised as having eliminated endemic measles transmission in 2014. Maintaining laboratory support for surveillance of vaccine-preventable diseases, such as measles, is an essential component of reaching and maintaining transmission-free status. Methodology: Real-time and conventional PCR-based tools were used to detect, differentiate from measles vaccine virus (MeVV), and sequence fragments of measles viruses (MeV) identified from specimens collected in Queensland. Specimens were mostly from travellers who had visited or returned to Queensland from international or interstate sites or been in contact with a case from either group. Results: Between 2010 and 2017, 13 678 specimens were tested in our laboratory using real-time RT-PCR (RT-rPCR), identifying 533 positives. Most specimens were swabs (70.98 %) and urines (25.56 %). A MeVV RT-rPCR was used on request and identified 154 instances of MeVV. MeV-positive extracts were genotyped as required. Genotypes identified among sequenced specimens included B3, D4, D8, D9, G3, and H1 as well as members of clade A as expected from the detection of MeV among virus introductions due to global travel and vaccination. Conclusion: We describe the workflow employed and results from our laboratory between 2010 and 2017 for the sensitive detection of MeV infection, supporting high-quality surveillance to ensure the maintenance of Australia's measles-free status. Competing Interests: The authors declare that there are no conflicts of interest. (© 2020 The Authors.) |
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| فهرسة مساهمة: | Keywords: Measles virus; diagnostics; epidemiology; measles |
| سلسلة جزيئية: | figshare 10.6084/m9.figshare.8248082 |
| تواريخ الأحداث: | Date Created: 20200925 Latest Revision: 20240329 |
| رمز التحديث: | 20240329 |
| مُعرف محوري في PubMed: | PMC7470308 |
| DOI: | 10.1099/acmi.0.000093 |
| PMID: | 32974570 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2516-8290 |
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| DOI: | 10.1099/acmi.0.000093 |