دورية أكاديمية
Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation.
العنوان: | Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation. |
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المؤلفون: | Alexander MR; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., Rootes CL; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., van Vuren PJ; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., Stewart CR; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia. Electronic address: cameron.stewart@csiro.au. |
المصدر: | Journal of virological methods [J Virol Methods] 2020 Dec; Vol. 286, pp. 113977. Date of Electronic Publication: 2020 Sep 23. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
مواضيع طبية MeSH: | Betacoronavirus/*isolation & purification , Clinical Laboratory Techniques/*methods , Coronavirus Infections/*virology , Pneumonia, Viral/*virology , Polyethylene Glycols/*chemistry, Animals ; COVID-19 ; COVID-19 Testing ; Caco-2 Cells ; Chlorocebus aethiops ; Coronavirus Infections/diagnosis ; Humans ; Pandemics ; Pneumonia, Viral/diagnosis ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Ultracentrifugation/methods ; Vero Cells |
مستخلص: | The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a ∼1.5 log (Crown Copyright © 2020. Published by Elsevier B.V. All rights reserved.) |
References: | J Hyg (Lond). 1972 Jun;70(2):255-65. (PMID: 4503868) J Virol. 2013 Apr;87(7):3782-91. (PMID: 23345523) Virology. 1970 Mar;40(3):734-44. (PMID: 4908735) J Gen Virol. 1979 Apr;43(1):247-52. (PMID: 113494) Am J Trop Med Hyg. 1969 Jul;18(4):588-91. (PMID: 5795451) J Virol Methods. 2008 Feb;147(2):328-32. (PMID: 18029032) Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387) mBio. 2015 Nov 10;6(6):e01697-15. (PMID: 26556276) N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945) Lancet Microbe. 2020 May;1(1):e14-e23. (PMID: 32835326) J Travel Med. 2020 Mar 13;27(2):. (PMID: 32052841) |
فهرسة مساهمة: | Keywords: COVID-19; PEG precipitation; SARS-CoV-2; Virus concentration |
المشرفين على المادة: | 3WJQ0SDW1A (Polyethylene Glycols) |
تواريخ الأحداث: | Date Created: 20200926 Date Completed: 20201130 Latest Revision: 20221221 |
رمز التحديث: | 20221221 |
مُعرف محوري في PubMed: | PMC7510776 |
DOI: | 10.1016/j.jviromet.2020.113977 |
PMID: | 32979405 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1879-0984 |
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DOI: | 10.1016/j.jviromet.2020.113977 |