دورية أكاديمية
أعرض في EDS

Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation.

التفاصيل البيبلوغرافية
العنوان: Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation.
المؤلفون: Alexander MR; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., Rootes CL; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., van Vuren PJ; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia., Stewart CR; CSIRO Health & Biosecurity, Australian Centre for Disease Preparedness, Private Bag 24, Geelong 3220, Victoria, Australia. Electronic address: cameron.stewart@csiro.au.
المصدر: Journal of virological methods [J Virol Methods] 2020 Dec; Vol. 286, pp. 113977. Date of Electronic Publication: 2020 Sep 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
مواضيع طبية MeSH: Betacoronavirus/*isolation & purification , Clinical Laboratory Techniques/*methods , Coronavirus Infections/*virology , Pneumonia, Viral/*virology , Polyethylene Glycols/*chemistry, Animals ; COVID-19 ; COVID-19 Testing ; Caco-2 Cells ; Chlorocebus aethiops ; Coronavirus Infections/diagnosis ; Humans ; Pandemics ; Pneumonia, Viral/diagnosis ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Ultracentrifugation/methods ; Vero Cells
مستخلص: The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a ∼1.5 log 10 increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas.
(Crown Copyright © 2020. Published by Elsevier B.V. All rights reserved.)
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فهرسة مساهمة: Keywords: COVID-19; PEG precipitation; SARS-CoV-2; Virus concentration
المشرفين على المادة: 3WJQ0SDW1A (Polyethylene Glycols)
تواريخ الأحداث: Date Created: 20200926 Date Completed: 20201130 Latest Revision: 20221221
رمز التحديث: 20221221
مُعرف محوري في PubMed: PMC7510776
DOI: 10.1016/j.jviromet.2020.113977
PMID: 32979405
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0984
DOI:10.1016/j.jviromet.2020.113977