دورية أكاديمية
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Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal rats.

التفاصيل البيبلوغرافية
العنوان: Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal rats.
المؤلفون: Kang JB; Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828 South Korea., Park DJ; Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828 South Korea., Son HK; Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828 South Korea., Koh PO; Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828 South Korea.
المصدر: Laboratory animal research [Lab Anim Res] 2020 Sep 18; Vol. 36, pp. 34. Date of Electronic Publication: 2020 Sep 18 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central, Ltd Country of Publication: England NLM ID: 101560684 Publication Model: eCollection Cited Medium: Print ISSN: 1738-6055 (Print) Linking ISSN: 17386055 NLM ISO Abbreviation: Lab Anim Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2019-: London : BioMed Central, Ltd.
Original Publication: Chungbuk : Korean Association for Laboratory Animal Science
مستخلص: Glutamate induces neurotoxicity during brain development, causing nerve damage. Protein phosphatase 2A (PP2A) is a type of serine/threonine phosphatase that regulates various biological functions. Among the PP2A subunit types, subunit B is abundant in brain tissue and plays an essential role in the nervous system. This study investigated changes in PP2A subunit B expression through glutamate exposure in the cerebral cortex of newborn rats. Sprague-Dawley rat pups (7 days after birth) were injected intraperitoneally with vehicle or glutamate (10 mg/kg). After 4 h of drug treatment, the brain tissue was isolated and fixed for morphological study. In addition, the cerebral cortex was collected for RNA and protein works. We observed severe histopathological changes including swollen neuron and atrophied dendrite in the glutamate exposed cerebral cortex. Glutamate exposure leads to a decrease in PP2A subunit B. Reverse-transcription PCR and Western blot analyses confirmed that glutamate induces a decrease of PP2A subunit B in the cerebral cortex of newborn rats. Moreover, immunohistochemical study showed a decrease in PP2A subunit B positive cells. The reduction of PP2A subunit B expression is considered an indicator of neurodegenerative damage. These results suggest that glutamate exposure causes neuronal damage in the cerebral cortex of new born rats through a decrease in PP2A subunit B.
Competing Interests: Competing interestsThe authors declare that there is no financial conflict of interests to publish these results.
(© The Author(s) 2020.)
References: Acta Pharmacol Sin. 2009 Apr;30(4):379-87. (PMID: 19343058)
J Neurosci. 1996 Oct 1;16(19):6125-33. (PMID: 8815895)
J Biol Chem. 1994 Aug 5;269(31):20139-48. (PMID: 8051102)
Trends Biochem Sci. 1999 May;24(5):186-91. (PMID: 10322434)
Int J Biochem Cell Biol. 2010 Nov;42(11):1775-8. (PMID: 20678581)
Biochem J. 1999 Apr 15;339 ( Pt 2):241-6. (PMID: 10191253)
J Vet Med Sci. 2007 Mar;69(3):285-8. (PMID: 17409645)
Psychopharmacology (Berl). 1993;111(4):391-401. (PMID: 7870979)
FEBS J. 2010 Sep;277(18):3622-36. (PMID: 20659161)
Behav Brain Res. 2003 Mar 18;140(1-2):1-47. (PMID: 12644276)
J Neurosci. 1996 Oct 15;16(20):6394-401. (PMID: 8815918)
Biochemistry. 1987 Nov 17;26(23):7215-20. (PMID: 2827745)
PLoS One. 2013;8(1):e54217. (PMID: 23349830)
Prog Neurobiol. 1998 Mar;54(4):369-415. (PMID: 9522394)
J Comp Neurol. 1998 Mar 23;392(4):515-27. (PMID: 9514514)
Pathophysiology. 2009 Oct;16(4):273-7. (PMID: 19278841)
Neural Plast. 2016;2016:7607924. (PMID: 27195153)
Pflugers Arch. 2010 Jul;460(2):525-42. (PMID: 20229265)
Biochem J. 2001 Feb 1;353(Pt 3):417-39. (PMID: 11171037)
J Biol Chem. 2000 Feb 25;275(8):5535-44. (PMID: 10681533)
Biochim Biophys Acta. 1999 May 5;1411(2-3):401-14. (PMID: 10320672)
Neuron. 1996 Dec;17(6):1201-7. (PMID: 8982166)
Biochem Pharmacol. 2000 Oct 15;60(8):1225-35. (PMID: 11007961)
Ann Clin Transl Neurol. 2016 Sep 07;3(10):769-780. (PMID: 27752512)
Lab Anim Res. 2011 Mar;27(1):73-6. (PMID: 21826165)
Proteomics. 2007 Aug;7(15):2624-35. (PMID: 17610204)
Cancer Res. 2019 Aug 15;79(16):4242-4257. (PMID: 31142515)
Cell Signal. 2001 Jan;13(1):7-16. (PMID: 11257442)
J Vet Med Sci. 2019 Jul 19;81(7):1047-1054. (PMID: 31092742)
Lab Anim Res. 2019 Nov 26;35:24. (PMID: 32257912)
فهرسة مساهمة: Keywords: Cerebral cortex; Glutamate; Neonate; PP2A
تواريخ الأحداث: Date Created: 20200930 Latest Revision: 20201002
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7501672
DOI: 10.1186/s42826-020-00064-y
PMID: 32995332
قاعدة البيانات: MEDLINE
الوصف
تدمد:1738-6055
DOI:10.1186/s42826-020-00064-y