دورية أكاديمية
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Identification and characterization of a sulfite reductase gene and new insights regarding the sulfur-containing amino acid metabolism in the basidiomycetous yeast Cryptococcus neoformans.

التفاصيل البيبلوغرافية
العنوان: Identification and characterization of a sulfite reductase gene and new insights regarding the sulfur-containing amino acid metabolism in the basidiomycetous yeast Cryptococcus neoformans.
المؤلفون: Nguyen PT; Department of Biological Science and Technology, Tokyo University of Science, Niijuku 6-3-1, Katsushika, Tokyo, 125-8585, Japan.; Southern Institute of Ecology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam., Toh-E A; Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chiba, 260-8673, Japan., Nguyen NH; Department of Biological Science and Technology, Tokyo University of Science, Niijuku 6-3-1, Katsushika, Tokyo, 125-8585, Japan.; Southern Institute of Ecology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam., Imanishi-Shimizu Y; College of Science and Engineering, Kanto Gakuin University, Mutsuura-higashi 1-50-1, Kanazawa-ku, Yokohama, Kanagawa, 236-8501, Japan., Watanabe A; Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chiba, 260-8673, Japan., Kamei K; Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chiba, 260-8673, Japan., Shimizu K; Department of Biological Science and Technology, Tokyo University of Science, Niijuku 6-3-1, Katsushika, Tokyo, 125-8585, Japan. shimizuk@rs.tus.ac.jp.; Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chiba, 260-8673, Japan. shimizuk@rs.tus.ac.jp.
المصدر: Current genetics [Curr Genet] 2021 Feb; Vol. 67 (1), pp. 115-128. Date of Electronic Publication: 2020 Oct 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: United States NLM ID: 8004904 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0983 (Electronic) Linking ISSN: 01728083 NLM ISO Abbreviation: Curr Genet Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Springer International
Original Publication: [New York] Springer International.
مواضيع طبية MeSH: Cryptococcus neoformans/*genetics , Cysteine/*genetics , Oxidoreductases Acting on Sulfur Group Donors/*genetics , Sulfur/*metabolism, Amino Acids/biosynthesis ; Amino Acids/metabolism ; Cryptococcus neoformans/metabolism ; Cysteine/metabolism ; Humans ; Hydrogen Sulfide/metabolism ; Methionine/genetics ; Methionine/metabolism ; Oxidoreductases Acting on Sulfur Group Donors/metabolism ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Saccharomyces cerevisiae/enzymology ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Sulfite Reductase (NADPH)/genetics ; Threonine-tRNA Ligase/genetics
مستخلص: The amino acid biosynthetic pathway of invasive pathogenic fungi has been studied as a potential antifungal drug target. Studies of the disruption of genes involved in amino acid biosynthesis have demonstrated the importance of this pathway in the virulence of Cryptococcus neoformans. Here, we identified the MET5 (CNL05500) and MET10 (CNG03990) genes in this pathway, both encoding sulfite reductase, which catalyzes the reduction of sulfite to sulfide. The MET14 (CNE03880) gene was also identified, which is responsible for the conversion of sulfate to sulfite. The use of cysteine as a sulfur source led to the production of methionine via hydrogen sulfide synthesis mediated by CYS4 (CNA06170), CYS3 (CNN01730), and MST1 (CND03690). MST1 exhibited high homology with the TUM1 gene of Saccharomyces cerevisiae, which has functional similarity with the 3-mercaptopyruvate sulfurtransferase (3-MST) gene in humans. Although the hypothesis that hydrogen sulfide is produced from cysteine via CYS4, CYS3, and MST1 warrants further study, the new insight into the metabolic pathway of sulfur-containing amino acids in C. neoformans provided here indicates the usefulness of this system in the development of screening tools for antifungal drug agents.
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معلومات مُعتمدة: 16K00661 Japan Society for the Promotion of Science; 19K123891 Japan Society for the Promotion of Science
فهرسة مساهمة: Keywords: Cystathionine; Cysteine; Hydrogen sulfide; Sulfate; Transsulfuration pathway
المشرفين على المادة: 0 (Amino Acids)
0 (Saccharomyces cerevisiae Proteins)
70FD1KFU70 (Sulfur)
AE28F7PNPL (Methionine)
EC 1.8.- (Oxidoreductases Acting on Sulfur Group Donors)
EC 1.8.1.2 (Sulfite Reductase (NADPH))
EC 1.8.1.2 (sulfite reductase (NADPH), S cerevisiae)
EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
EC 2.7.1.25 (adenylylsulfate kinase)
EC 6.1.1.3 (MST1 protein, S cerevisiae)
EC 6.1.1.3 (Threonine-tRNA Ligase)
K848JZ4886 (Cysteine)
YY9FVM7NSN (Hydrogen Sulfide)
تواريخ الأحداث: Date Created: 20201001 Date Completed: 20210706 Latest Revision: 20210706
رمز التحديث: 20231215
DOI: 10.1007/s00294-020-01112-9
PMID: 33001274
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0983
DOI:10.1007/s00294-020-01112-9