دورية أكاديمية
Mixotrophic Iron-Oxidizing Thiomonas Isolates from an Acid Mine Drainage-Affected Creek.
| العنوان: | Mixotrophic Iron-Oxidizing Thiomonas Isolates from an Acid Mine Drainage-Affected Creek. |
|---|---|
| المؤلفون: | Akob DM; U.S. Geological Survey, Reston, Virginia, USA dakob@usgs.gov cschan@udel.edu., Hallenbeck M; Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.; Delaware Biotechnology Institute, Newark, Delaware, USA., Beulig F; Institute of Biodiversity, Friedrich Schiller University Jena, Jena, Germany., Fabisch M; Institute of Biodiversity, Friedrich Schiller University Jena, Jena, Germany., Küsel K; Institute of Biodiversity, Friedrich Schiller University Jena, Jena, Germany., Keffer JL; Department of Earth Sciences, University of Delaware, Newark, Delaware, USA.; Delaware Biotechnology Institute, Newark, Delaware, USA., Woyke T; Joint Genome Institute, U.S. Department of Energy, Berkeley, California, USA., Shapiro N; Joint Genome Institute, U.S. Department of Energy, Berkeley, California, USA., Lapidus A; Joint Genome Institute, U.S. Department of Energy, Berkeley, California, USA.; Center for Algorithmic Biotechnology, St. Petersburg State University, St. Petersburg, Russia., Klenk HP; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom., Chan CS; Department of Biological Sciences, University of Delaware, Newark, Delaware, USA dakob@usgs.gov cschan@udel.edu.; Department of Earth Sciences, University of Delaware, Newark, Delaware, USA.; Delaware Biotechnology Institute, Newark, Delaware, USA. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2020 Nov 24; Vol. 86 (24). Date of Electronic Publication: 2020 Nov 24 (Print Publication: 2020). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Burkholderiales/*metabolism , Iron/*metabolism , Rivers/*microbiology , Wastewater/*microbiology, Germany ; Mining ; Oxidation-Reduction |
| مستخلص: | Natural attenuation of heavy metals occurs via coupled microbial iron cycling and metal precipitation in creeks impacted by acid mine drainage (AMD). Here, we describe the isolation, characterization, and genomic sequencing of two iron-oxidizing bacteria (FeOB) species: Thiomonas ferrovorans FB-6 and Thiomonas metallidurans FB-Cd, isolated from slightly acidic (pH 6.3), Fe-rich, AMD-impacted creek sediments. These strains precipitated amorphous iron oxides, lepidocrocite, goethite, and magnetite or maghemite and grew at a pH optimum of 5.5. While Thiomonas spp. are known as mixotrophic sulfur oxidizers and As oxidizers, the FB strains oxidized Fe, which suggests they can efficiently remove Fe and other metals via coprecipitation. Previous evidence for Thiomonas sp. Fe oxidation is largely ambiguous, possibly because of difficulty demonstrating Fe oxidation in heterotrophic/mixotrophic organisms. Therefore, we also conducted a genomic analysis to identify genetic mechanisms of Fe oxidation, other metal transformations, and additional adaptations, comparing the two FB strain genomes with 12 other Thiomonas genomes. The FB strains fall within a relatively novel group of Thiomonas strains that includes another strain (b6) with solid evidence of Fe oxidation. Most Thiomonas isolates, including the FB strains, have the putative iron oxidation gene cyc2 , but only the two FB strains possess the putative Fe oxidase genes mtoAB The two FB strain genomes contain the highest numbers of strain-specific gene clusters, greatly increasing the known Thiomonas genetic potential. Our results revealed that the FB strains are two distinct novel species of Thiomonas with the genetic potential for bioremediation of AMD via iron oxidation. IMPORTANCE As AMD moves through the environment, it impacts aquatic ecosystems, but at the same time, these ecosystems can naturally attenuate contaminated waters via acid neutralization and catalyzing metal precipitation. This is the case in the former Ronneburg uranium-mining district, where AMD impacts creek sediments. We isolated and characterized two iron-oxidizing Thiomonas species that are mildly acidophilic to neutrophilic and that have two genetic pathways for iron oxidation. These Thiomonas species are well positioned to naturally attenuate AMD as it discharges across the landscape. (Copyright © 2020 American Society for Microbiology.) |
| References: | Microb Biotechnol. 2017 Jul;10(4):789-803. (PMID: 28169492) Environ Sci Technol. 2010 Jan 1;44(1):94-101. (PMID: 20039738) Nucleic Acids Res. 2004 Feb 25;32(4):1363-71. (PMID: 14985472) Environ Sci Pollut Res Int. 2018 Jan;25(2):1470-1483. (PMID: 29090447) PeerJ. 2018 Jan 25;6:e4320. (PMID: 29423345) Sci Total Environ. 2005 Jun 1;345(1-3):219-28. (PMID: 15919541) Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2816-2821. (PMID: 21216915) Proc Natl Acad Sci U S A. 2016 May 10;113(19):5441-6. (PMID: 27114545) J Biol Chem. 2008 Sep 19;283(38):25803-11. (PMID: 18632666) Int J Syst Bacteriol. 1997 Apr;47(2):522-8. (PMID: 9103643) Microbiology (Reading). 2011 Jan;157(Pt 1):111-122. (PMID: 20884692) Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2553-2557. (PMID: 17082389) Front Microbiol. 2015 Apr 28;6:332. (PMID: 25972843) Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2720-2724. (PMID: 17978245) Biochem Soc Trans. 2012 Dec 1;40(6):1261-7. (PMID: 23176465) Extremophiles. 2010 Jan;14(1):9-19. (PMID: 19787416) J Bacteriol. 1951 Nov;62(5):605-11. (PMID: 14897836) Environ Microbiol. 2010 Oct;12(10):2814-25. (PMID: 20545739) J Bacteriol. 1991 Jan;173(2):697-703. (PMID: 1987160) Methods Enzymol. 2005;397:112-23. (PMID: 16260287) Nucleic Acids Res. 2016 Jan 4;44(D1):D7-19. (PMID: 26615191) Sci Total Environ. 2019 Jan 1;646:972-988. (PMID: 30235650) Microbiol Mol Biol Rev. 2009 Sep;73(3):510-28, Table of Contents. (PMID: 19721088) ISME J. 2011 Apr;5(4):717-27. (PMID: 21107443) FEMS Microbiol Ecol. 2019 Apr 1;95(4):. (PMID: 30874727) J Gen Microbiol. 1993 Jul;139(7):1531-5. (PMID: 8371116) mSystems. 2020 Feb 18;5(1):. (PMID: 32071158) Front Microbiol. 2018 Dec 21;9:3169. (PMID: 30627121) Antonie Van Leeuwenhoek. 2006 Jan;89(1):99-108. (PMID: 16341463) Environ Microbiol. 2008 Jan;10(1):228-37. (PMID: 17894815) Biol Direct. 2012 Sep 10;7:28. (PMID: 22963335) Appl Environ Microbiol. 1997 Dec;63(12):4784-92. (PMID: 9406396) Appl Environ Microbiol. 2015 Sep 1;81(17):5927-37. (PMID: 26092463) BMC Microbiol. 2009 Jun 23;9:127. (PMID: 19549320) Bioinformatics. 2012 Jun 15;28(12):1647-9. (PMID: 22543367) BMC Genomics. 2008 Feb 08;9:75. (PMID: 18261238) Appl Microbiol Biotechnol. 2017 Feb;101(3):1239-1252. (PMID: 27832308) Front Microbiol. 2013 Sep 12;4:254. (PMID: 24062729) Geobiology. 2016 Jan;14(1):68-90. (PMID: 26407813) Arch Microbiol. 2011 Jun;193(6):439-49. (PMID: 21409355) Front Microbiol. 2017 Jul 18;8:1280. (PMID: 28769885) J Appl Microbiol. 2002;93(4):656-67. (PMID: 12234349) Appl Environ Microbiol. 1990 Feb;56(2):315-22. (PMID: 16348110) J Bacteriol. 2002 Jan;184(1):313-7. (PMID: 11741873) FEMS Microbiol Ecol. 2003 May 1;44(2):139-52. (PMID: 19719632) Lett Appl Microbiol. 2004;39(6):495-503. (PMID: 15548301) Geochem Trans. 2004 Jun 30;5(2):13. (PMID: 35412773) Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2171-5. (PMID: 19605731) FEMS Microbiol Ecol. 2001 Jan;34(3):181-186. (PMID: 11137597) PLoS One. 2015 Sep 30;10(9):e0139011. (PMID: 26422469) Front Cell Dev Biol. 2016 Feb 01;4:3. (PMID: 26870729) Methods Mol Biol. 2007;369:449-66. (PMID: 17656764) Front Microbiol. 2012 Feb 08;3:37. (PMID: 22347878) Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1513-8. (PMID: 21187386) J Mol Biol. 2016 Feb 22;428(4):726-731. (PMID: 26585406) J Bacteriol. 2014 Dec;196(24):4206-15. (PMID: 25182500) Front Microbiol. 2013 Dec 18;4:390. (PMID: 24385973) Nucleic Acids Res. 2019 Jan 8;47(D1):D666-D677. (PMID: 30289528) Bioinformatics. 2006 Nov 1;22(21):2688-90. (PMID: 16928733) PLoS Genet. 2010 Feb 26;6(2):e1000859. (PMID: 20195515) Front Microbiol. 2015 Sep 28;6:993. (PMID: 26441922) Microb Ecol. 2002 May;43(4):424-31. (PMID: 11953812) ISME J. 2017 Nov;11(11):2399-2406. (PMID: 28731467) Appl Environ Microbiol. 2010 Dec;76(24):8174-83. (PMID: 20971876) PLoS One. 2011;6(9):e25386. (PMID: 21966516) Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W394-9. (PMID: 16845035) Appl Environ Microbiol. 2006 Mar;72(3):2022-30. (PMID: 16517651) Nat Methods. 2013 Jun;10(6):563-9. (PMID: 23644548) Appl Environ Microbiol. 2002 Jun;68(6):2704-10. (PMID: 12039723) Front Microbiol. 2015 Nov 13;6:1265. (PMID: 26617599) J Exp Bot. 2008;59(7):1525-41. (PMID: 18245799) J Appl Microbiol. 2003;95(3):492-9. (PMID: 12911697) PLoS One. 2007 Aug 01;2(7):e667. (PMID: 17668050) PeerJ. 2015 Oct 08;3:e1319. (PMID: 26500826) Appl Environ Microbiol. 1985 Jan;49(1):1-7. (PMID: 3883894) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) FEMS Microbiol Lett. 2016 May;363(10):. (PMID: 27036143) Front Microbiol. 2017 Aug 21;8:1584. (PMID: 28871245) Mol Microbiol. 2006 May;60(3):563-77. (PMID: 16629661) Water Res. 2003 Jul;37(12):2929-36. (PMID: 12767295) Science. 2009 Jan 2;323(5910):133-8. (PMID: 19023044) |
| فهرسة مساهمة: | Keywords: Thiomonas; acid mine drainage; cyc2; heavy metals; iron oxidation; pangenome |
| المشرفين على المادة: | 0 (Waste Water) E1UOL152H7 (Iron) |
| تواريخ الأحداث: | Date Created: 20201003 Date Completed: 20201221 Latest Revision: 20240330 |
| رمز التحديث: | 20240330 |
| مُعرف محوري في PubMed: | PMC7688216 |
| DOI: | 10.1128/AEM.01424-20 |
| PMID: | 33008825 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
|---|---|
| DOI: | 10.1128/AEM.01424-20 |