دورية أكاديمية
Determining Theileria annulata parasitemia through qPCR in lactating cows of Odisha, - a theileriosis-endemic region of India.
| العنوان: | Determining Theileria annulata parasitemia through qPCR in lactating cows of Odisha, - a theileriosis-endemic region of India. |
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| المؤلفون: | Sahoo S; Department of Veterinary Epidemiology and Preventive Medicine, Odisha University of Agriculture and Technology, Bhubaneswar, India., Sahoo N; Department of Veterinary Epidemiology and Preventive Medicine, Odisha University of Agriculture and Technology, Bhubaneswar, India.; Centre for Wildlife Health, Odisha University of Agriculture and Technology, Bhubaneswar, India., Biswal S; Department of Veterinary Epidemiology and Preventive Medicine, Odisha University of Agriculture and Technology, Bhubaneswar, India., Mohanty B; Department of Veterinary Parasitology, Odisha University of Agriculture and Technology, Bhubaneswar, India., Behera B; Centre for Wildlife Health, Odisha University of Agriculture and Technology, Bhubaneswar, India., Pahari A; Centre for Wildlife Health, Odisha University of Agriculture and Technology, Bhubaneswar, India., Bhuyan K; Department of Veterinary Epidemiology and Preventive Medicine, Odisha University of Agriculture and Technology, Bhubaneswar, India. |
| المصدر: | Animal biotechnology [Anim Biotechnol] 2022 Jun; Vol. 33 (3), pp. 487-492. Date of Electronic Publication: 2020 Oct 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Abingdon, Oxford : Taylor & Francis Original Publication: New York, N.Y. : Marcel Dekker, c1990- |
| مواضيع طبية MeSH: | Cattle Diseases*/epidemiology , Theileria annulata*/genetics , Theileriasis*/diagnosis , Theileriasis*/epidemiology, Animals ; Cattle ; Female ; Lactation ; Parasitemia/epidemiology ; Parasitemia/veterinary ; Phylogeny ; Real-Time Polymerase Chain Reaction/veterinary |
| مستخلص: | Study was undertaken in a theileriosis-endemic region of India during May 2018 to April 2019 among milch cows. Blood samples collected from apparently healthy ( n = 65) and Theileria-suspect cows ( n = 65) were screened against T. annulata and T. orientalis infection by SYBR Green‒based real time PCR using primers designed from the isolates of study area. Cows having single infection with T. annulata with/without clinical signs of inappetence, low milk yield, pale mucous membranes, fever, enlarged prescapular lymph node, soil licking, panting, coughing, salivation and lachrymation were subjected to further investigation where parasitaemia and piroplasms per 1000 erythrocytes ranged from 1.6 × 10 7 to 1.2 × 10 8 parasites/mL of blood and 3-24 piroplasms in moderate group (16/65), 4.4 × 10 8 to 6.9 × 10 9 parasites/mL of blood and >88 piroplasms in severe group (30/65) and 1.6 × 10 4 to 5.5 × 10 6 parasites/mL of blood and 0-1 piroplasms in asymptomatic or carriers (17/65), respectively. Study unfolded significant difference in T. annulata parasitaemia among apparently healthy and ill cows. Phylogenetic analysis of our T. annulata isolates (NCBI accession numbers MN098316, MN098317 and MN098318) exhibited maximum similarity with the isolates detected in other parts of India. |
| فهرسة مساهمة: | Keywords: T. annulata; carriers; milch cows; parasitemia |
| تواريخ الأحداث: | Date Created: 20201007 Date Completed: 20220602 Latest Revision: 20220602 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/10495398.2020.1806859 |
| PMID: | 33026945 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2378 |
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| DOI: | 10.1080/10495398.2020.1806859 |