دورية أكاديمية
Characterization of the Altai Maral Chymosin Gene, Production of a Chymosin Recombinant Analog in the Prokaryotic Expression System, and Analysis of Its Several Biochemical Properties.
العنوان: | Characterization of the Altai Maral Chymosin Gene, Production of a Chymosin Recombinant Analog in the Prokaryotic Expression System, and Analysis of Its Several Biochemical Properties. |
---|---|
المؤلفون: | Belenkaya SV; State Research Center of Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk Region, 630559, Russia. belenkaya.sveta@gmail.com.; Novosibirsk State University, Novosibirsk, 630090, Russia., Bondar AA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Kurgina TA; Novosibirsk State University, Novosibirsk, 630090, Russia.; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Elchaninov VV; Federal Altai Scientific Center of Agrobiotechnologies, Siberian Research Institute of Cheese Making, Barnaul, 656910, Russia., Bakulina AY; State Research Center of Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk Region, 630559, Russia.; Novosibirsk State University, Novosibirsk, 630090, Russia., Rukhlova EA; Novosibirsk State University, Novosibirsk, 630090, Russia., Lavrik OI; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Ilyichev AA; State Research Center of Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk Region, 630559, Russia., Shcherbakov DN; State Research Center of Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk Region, 630559, Russia.; Altai State University, Barnaul, 656049, Russia. |
المصدر: | Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2020 Jul; Vol. 85 (7), pp. 781-791. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MAIK Nauka/Interperiodica Country of Publication: United States NLM ID: 0376536 Publication Model: Print Cited Medium: Internet ISSN: 1608-3040 (Electronic) Linking ISSN: 00062979 NLM ISO Abbreviation: Biochemistry (Mosc) Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2007->: Moscow : MAIK Nauka/Interperiodica Original Publication: New York, Consultants Bureau [etc.] |
مواضيع طبية MeSH: | Chymosin/*genetics , Chymosin/*metabolism , Deer/*genetics, Animals ; Base Sequence ; Chymosin/chemistry ; Deer/metabolism ; Recombinant Proteins/chemistry |
مستخلص: | For the first time, the chymosin gene (CYM) of a maral was characterized. Its exon/intron organization was established using comparative analysis of the nucleotide sequence. The CYM mRNA sequence encoding a maral preprochymosin was reconstructed. Nucleotide sequence of the CYM maral mRNA allowed developing an expression vector to ensure production of a recombinant enzyme. Recombinant maral prochymosin was obtained in the expression system of Escherichia coli [strain BL21 (DE3)]. Total milk-coagulation activity (MCA) of the recombinant maral chymosin was 2330 AU/ml. The recombinant maral prochymosin relative activity was 52955 AU/mg. The recombinant maral chymosin showed 100-81% MCA in the temperature range 30-50°C, thermal stability (TS) threshold was 50°C, and the enzyme was completely inactivated at 70°C. Preparations of the recombinant chymosin of a single-humped camel and recombinant bovine chymosin were used as reference samples. Michaelis-Menten constant (K |
المشرفين على المادة: | 0 (Recombinant Proteins) EC 3.4.23.4 (Chymosin) |
تواريخ الأحداث: | Date Created: 20201012 Date Completed: 20210208 Latest Revision: 20210208 |
رمز التحديث: | 20231215 |
DOI: | 10.1134/S0006297920070068 |
PMID: | 33040722 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1608-3040 |
---|---|
DOI: | 10.1134/S0006297920070068 |