دورية أكاديمية
CD38 triggers inflammasome-mediated pyroptotic cell death in head and neck squamous cell carcinoma.
| العنوان: | CD38 triggers inflammasome-mediated pyroptotic cell death in head and neck squamous cell carcinoma. |
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| المؤلفون: | Zhang MJ; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Gao W; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Liu S; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Siu SP; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Yin M; Department of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical University Nanjing 210029, China., Ng JC; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Chow VL; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Chan JY; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China., Wong TS; Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. |
| المصدر: | American journal of cancer research [Am J Cancer Res] 2020 Sep 01; Vol. 10 (9), pp. 2895-2908. Date of Electronic Publication: 2020 Sep 01 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: e-Century Pub. Corp Country of Publication: United States NLM ID: 101549944 Publication Model: eCollection Cited Medium: Print ISSN: 2156-6976 (Print) Linking ISSN: 21566976 NLM ISO Abbreviation: Am J Cancer Res Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Madison, WI : e-Century Pub. Corp., [2011]- |
| مستخلص: | Background: Pyroptosis is a form of inflammatory cell death. Although it is recognized that NLRP3 (nucleotide-binding domain, leucine-rich repeat-containing family, pyrin domain-containing 3) inflammasome is involved in pyroptosis activation, the mechanism by which head and neck squamous cell carcinoma (HNSCC) inhibits pyroptotic cell death remains undefined. This study aims to delineate the role of calcium regulator CD38 in NLRP3 inflammasome-dependent pyroptosis in HNSCC. Methods: CD38 overexpressing HNSCC cell lines (SAS, CAL27, SNU899) were generated using lentiviral vectors. NLRP3 and gasdermin D (GSDMD) quantity were detected using Western blot. Caspase-1 activity changes were measured using the Caspase-Glo ® 1 inflammasome assay. Cell death proportion was determined by flow cytometry analysis. Proliferation assay was performed using xCELLigence RTCA system. Mouse xenotransplantation was performed to evaluate the potential oncogenic or tumor-suppressive function of CD38. ChIP assay was conducted to verify whether transcription factor NFAT1-mediated NLRP3 expression. Results: Exogenous calcium treatment can lead to a significant increase in caspase-1 activity in HNSCC. This feature was also observed in HNSCC cells with stable CD38 overexpression. CD38-overexpressing cell lines showed a significant reduction in proliferation. Further, expression of NLRP3 protein level was significantly increased in CD38-overexpressing cell lines. The N-terminal effector domain of GSDMD was remarkably increased in the CD38-overexpressing HNSCC. ChIP assay indicated that calcium-sensitive transcription factor NFAT1 was possibly involved in the transcriptional upregulation of NLRP3 observed in CD38-overexpressing HNSCC. The pre-clinical xenograft model revealed that CD38 expression had an inhibiting function on HNSCC progression. Conclusion: In conclusion, our results suggested that activation of pyroptosis in HNSCC is a calcium-dependent process. Reduced expression of calcium ion regulator CD38 functions could prevent inflammasome-induced pyroptosis in HNSCC. CD38 may function as a tumor suppressor in HNSCC progression. Competing Interests: None. (AJCR Copyright © 2020.) |
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| فهرسة مساهمة: | Keywords: CD38; NLRP3; calcium; head and neck squamous cell carcinoma; pyroptosis |
| تواريخ الأحداث: | Date Created: 20201012 Latest Revision: 20201013 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7539785 |
| PMID: | 33042624 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2156-6976 |
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