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The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers.

التفاصيل البيبلوغرافية
العنوان: The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers.
المؤلفون: Sato TP; Department of Dental Materials and Prosthodontics, Institute of Science and Technology (IST), São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP, 12245-200, Brazil., Rodrigues BVM; Laboratory of Biomedical Nanotechnology, Universidade Brasil, São Paulo, SP, 08230-030, Brazil.; Plasma and Processes Laboratory, Technological Institute of Aeronautics (ITA-CTA), São José dos Campos, SP, 12228-900, Brazil., Mello DCR; Department of Oral Pathology and Microbiology, IST, UNESP, São José dos Campos, SP, 12245-200, Brazil., Münchow EA; Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil., Ribeiro JS; Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA., Machado JPB; Associated Laboratory of Materials and Sensor (LAS), National Institute of Space Research (INPE), São José dos Campos, SP, 12201-970, Brazil., Vasconcellos LMR; Department of Oral Pathology and Microbiology, IST, UNESP, São José dos Campos, SP, 12245-200, Brazil., Lobo AO; Laboratory of Biomedical Nanotechnology, Universidade Brasil, São Paulo, SP, 08230-030, Brazil.; Interdisciplinary Laboratory for Advanced Materials, Federal University of Piauí, Teresina, PI, 64049-550, Brazil., Bottino MC; Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA. mbottino@umich.edu., Borges ALS; Department of Dental Materials and Prosthodontics, Institute of Science and Technology (IST), São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP, 12245-200, Brazil. alexandre.borges@unesp.br.
المصدر: Clinical oral investigations [Clin Oral Investig] 2021 May; Vol. 25 (5), pp. 3095-3103. Date of Electronic Publication: 2020 Oct 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 9707115 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1436-3771 (Electronic) Linking ISSN: 14326981 NLM ISO Abbreviation: Clin Oral Investig
أسماء مطبوعة: Publication: Berlin : Springer-Verlag
Original Publication: Berlin : Springer, c1997-
مواضيع طبية MeSH: Chitosan*/pharmacology , Nanofibers*, Durapatite ; Polymers
مستخلص: Objectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers.
Materials and Methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey's test (α = 0.05).
Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm.
Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers.
Clinical Relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry.
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معلومات مُعتمدة: 2011/17877-7 and 2011/20345-7 FAPESP; 310973/2014-7 and 310659/2014-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico
فهرسة مساهمة: Keywords: Antimicrobial; Chitosan; Electrospinning; Regeneration; Regenerative dentistry; Scaffolds
المشرفين على المادة: 0 (Polymers)
9012-76-4 (Chitosan)
91D9GV0Z28 (Durapatite)
تواريخ الأحداث: Date Created: 20201013 Date Completed: 20210423 Latest Revision: 20210423
رمز التحديث: 20231215
DOI: 10.1007/s00784-020-03633-6
PMID: 33047204
قاعدة البيانات: MEDLINE
الوصف
تدمد:1436-3771
DOI:10.1007/s00784-020-03633-6