دورية أكاديمية
A Therapeutic Non-self-reactive SARS-CoV-2 Antibody Protects from Lung Pathology in a COVID-19 Hamster Model.
العنوان: | A Therapeutic Non-self-reactive SARS-CoV-2 Antibody Protects from Lung Pathology in a COVID-19 Hamster Model. |
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المؤلفون: | Kreye J; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Department of Pediatric Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany. Electronic address: jakob.kreye@dzne.de., Reincke SM; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Berlin Institute of Health (BIH), 10178 Berlin, Germany., Kornau HC; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Neuroscience Research Center (NWFZ), Cluster NeuroCure, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Sánchez-Sendin E; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Corman VM; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Liu H; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Yuan M; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Wu NC; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Zhu X; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Lee CD; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Trimpert J; Institute of Virology, Freie Universität Berlin, 14163 Berlin, Germany., Höltje M; Institute of Integrative Neuroanatomy Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Dietert K; Institute of Veterinary Pathology, Freie Universität Berlin, 14163 Berlin, Germany; Veterinary Centre for Resistance Research, Freie Universität Berlin, 14163 Berlin, Germany., Stöffler L; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., von Wardenburg N; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., van Hoof S; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Homeyer MA; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Berlin Institute of Health (BIH), 10178 Berlin, Germany., Hoffmann J; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Abdelgawad A; Institute of Virology, Freie Universität Berlin, 14163 Berlin, Germany., Gruber AD; Institute of Veterinary Pathology, Freie Universität Berlin, 14163 Berlin, Germany., Bertzbach LD; Institute of Virology, Freie Universität Berlin, 14163 Berlin, Germany., Vladimirova D; Institute of Virology, Freie Universität Berlin, 14163 Berlin, Germany., Li LY; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Institute of Integrative Neuroanatomy Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Barthel PC; Institute of Integrative Neuroanatomy Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Skriner K; Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Hocke AC; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Hippenstiel S; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Witzenrath M; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Suttorp N; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Kurth F; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine and I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20359 Hamburg, Germany., Franke C; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Endres M; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Center for Stroke Research Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; Excellence Cluster NeuroCure Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany; German Centre for Cardiovascular Research (DZHK), Partner Site Berlin, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10785 Berlin, Germany., Schmitz D; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Neuroscience Research Center (NWFZ), Cluster NeuroCure, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Jeworowski LM; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Richter A; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Schmidt ML; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Schwarz T; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Müller MA; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Drosten C; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany, and German Centre for Infection Research (DZIF), 10117 Berlin, Germany., Wendisch D; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Sander LE; Department of Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany., Osterrieder N; Institute of Virology, Freie Universität Berlin, 14163 Berlin, Germany; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong., Wilson IA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA; The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Prüss H; German Center for Neurodegenerative Diseases (DZNE) Berlin, 10117 Berlin, Germany; Helmholtz Innovation Lab BaoBab (Brain Antibody-omics and B-cell Lab), 10117 Berlin, Germany; Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 10117 Berlin, Germany. Electronic address: harald.pruess@dzne.de. |
المصدر: | Cell [Cell] 2020 Nov 12; Vol. 183 (4), pp. 1058-1069.e19. Date of Electronic Publication: 2020 Sep 23. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
مواضيع طبية MeSH: | Antibodies, Monoclonal/*immunology , Antibodies, Viral/*immunology , Betacoronavirus/*metabolism , Coronavirus Infections/*pathology , Pneumonia, Viral/*pathology, Angiotensin-Converting Enzyme 2 ; Animals ; Antibodies, Monoclonal/therapeutic use ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/therapeutic use ; Antigen-Antibody Reactions ; Betacoronavirus/immunology ; Betacoronavirus/pathogenicity ; Binding Sites ; COVID-19 ; Coronavirus Infections/drug therapy ; Coronavirus Infections/virology ; Cricetinae ; Crystallography, X-Ray ; Disease Models, Animal ; Humans ; Kinetics ; Lung/immunology ; Lung/metabolism ; Lung/pathology ; Mice ; Mice, Inbred C57BL ; Molecular Dynamics Simulation ; Pandemics ; Peptidyl-Dipeptidase A/chemistry ; Peptidyl-Dipeptidase A/metabolism ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/virology ; Protein Binding ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/immunology ; Spike Glycoprotein, Coronavirus/metabolism |
مستخلص: | The emergence of SARS-CoV-2 led to pandemic spread of coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization of virus-neutralizing antibodies and target epitopes is needed to understand COVID-19 pathophysiology and guide immunization strategies. Among 598 human monoclonal antibodies (mAbs) from 10 COVID-19 patients, we identified 40 strongly neutralizing mAbs. The most potent mAb, CV07-209, neutralized authentic SARS-CoV-2 with an IC Competing Interests: Declaration of Interests Related to this work, the German Center for Neurodegenerative Diseases (DZNE) and Charité-Universitätsmedizin Berlin have filed a patent application on which J.K., S.M.R., H.-C.K., E.S.-S., V.M.C., M.A.M., D.W., L.E.S., and H.P. are named as inventors. (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.) |
التعليقات: | Update of: bioRxiv. 2020 Aug 16;:. (PMID: 32817952) Comment in: Signal Transduct Target Ther. 2021 Mar 2;6(1):105. (PMID: 33654054) |
References: | Cell. 2020 Jul 9;182(1):73-84.e16. (PMID: 32425270) J Immunol Methods. 2011 May 31;368(1-2):24-35. (PMID: 21334343) Brain. 2016 Oct;139(Pt 10):2641-2652. (PMID: 27543972) PLoS Pathog. 2016 Aug 25;12(8):e1005815. (PMID: 27560183) Science. 2011 Aug 12;333(6044):843-50. (PMID: 21737702) PLoS Med. 2006 Jul;3(7):e237. (PMID: 16796401) J Immunol Methods. 2008 Jan 1;329(1-2):112-24. (PMID: 17996249) Science. 2020 Jun 12;368(6496):1274-1278. (PMID: 32404477) N Engl J Med. 2020 Jun 4;382(23):2268-2270. (PMID: 32294339) J Autoimmun. 2007 Dec;29(4):219-28. (PMID: 17888628) Nature. 2020 Aug;584(7821):437-442. (PMID: 32555388) Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507) Science. 2020 Aug 28;369(6507):1119-1123. (PMID: 32661058) J Comp Pathol. 2020 Jan;174:39-53. (PMID: 31955802) J Vet Med Sci. 2017 Jul 19;79(7):1230-1235. (PMID: 28603217) Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387) Cell. 2020 Aug 20;182(4):843-854.e12. (PMID: 32673567) N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945) Cell. 2020 Oct 15;183(2):429-441.e16. (PMID: 32941803) Infection. 2020 Aug;48(4):619-626. (PMID: 32535877) Science. 2020 Aug 7;369(6504):650-655. (PMID: 32571838) Nature. 2020 Aug;584(7821):450-456. (PMID: 32698192) Cell. 2020 Apr 16;181(2):281-292.e6. (PMID: 32155444) J Exp Med. 2011 Oct 24;208(11):2237-49. (PMID: 21987658) J Biol Chem. 2006 Jun 9;281(23):15829-36. (PMID: 16597622) Methods Enzymol. 1997;276:307-26. (PMID: 27754618) Science. 2020 Aug 7;369(6504):731-736. (PMID: 32540900) Nature. 2020 May;581(7809):465-469. (PMID: 32235945) Science. 2020 May 8;368(6491):630-633. (PMID: 32245784) BMC Bioinformatics. 2020 Oct 8;21(1):446. (PMID: 33032524) Bioinformatics. 2014 Oct;30(19):2811-2. (PMID: 24930139) Nature. 2020 Aug;584(7819):115-119. (PMID: 32454513) J Mol Biol. 2018 Feb 2;430(3):337-347. (PMID: 29273204) Science. 2020 Aug 21;369(6506):956-963. (PMID: 32540903) J Mol Biol. 2007 Sep 21;372(3):774-97. (PMID: 17681537) Science. 2020 Nov 27;370(6520):1110-1115. (PMID: 33037066) Am J Respir Cell Mol Biol. 2020 Dec;63(6):856-859. (PMID: 32897757) Nature. 2020 Aug;584(7821):443-449. (PMID: 32668443) Acta Crystallogr D Biol Crystallogr. 2006 Aug;62(Pt 8):859-66. (PMID: 16855301) Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765) PLoS One. 2017 Nov 20;12(11):e0188251. (PMID: 29155867) Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. (PMID: 20124702) Ann Neurol. 2020 Mar;87(3):405-418. (PMID: 31900946) Euro Surveill. 2013 Feb 21;18(8):. (PMID: 23449231) Nat Rev Immunol. 2016 Aug;16(8):498-508. (PMID: 27374636) Science. 2020 Aug 7;369(6504):643-650. (PMID: 32540902) Cell. 2020 Aug 20;182(4):828-842.e16. (PMID: 32645326) J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. (PMID: 19461840) Cell. 2020 Apr 16;181(2):271-280.e8. (PMID: 32142651) N Engl J Med. 2020 Jul 17;:. (PMID: 32678530) Viruses. 2020 Jul 20;12(7):. (PMID: 32698441) Nature. 2020 May;581(7807):215-220. (PMID: 32225176) Cell. 2019 Feb 21;176(5):1026-1039.e15. (PMID: 30712865) J Exp Med. 2013 Feb 11;210(2):241-56. (PMID: 23359068) J Exp Med. 2016 Jun 27;213(7):1255-65. (PMID: 27298445) |
معلومات مُعتمدة: | K99 AI139445 United States AI NIAID NIH HHS; P41 GM103393 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; autoreactivity; crystal structures; hamster model; monoclonal antibody; neutralizing antibody; post-exposure; self-antigens; self-reactivity |
المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) 0 (Spike Glycoprotein, Coronavirus) 0 (spike protein, SARS-CoV-2) EC 3.4.15.1 (Peptidyl-Dipeptidase A) EC 3.4.17.23 (ACE2 protein, human) EC 3.4.17.23 (Ace2 protein, mouse) EC 3.4.17.23 (Angiotensin-Converting Enzyme 2) |
تواريخ الأحداث: | Date Created: 20201015 Date Completed: 20201125 Latest Revision: 20240211 |
رمز التحديث: | 20240211 |
مُعرف محوري في PubMed: | PMC7510528 |
DOI: | 10.1016/j.cell.2020.09.049 |
PMID: | 33058755 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1097-4172 |
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DOI: | 10.1016/j.cell.2020.09.049 |