دورية أكاديمية
أعرض في EDS

Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret ( Mustela Nigripes ).

التفاصيل البيبلوغرافية
العنوان: Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret ( Mustela Nigripes ).
المؤلفون: Lima DBC; Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceará (Universidade Estadual do Ceará, UECE), 1700 Doutor Silas Munguba Avenue, Fortaleza, 0714-903 CE, Brazil., Silva LDMD; Laboratory of Carnivore Reproduction, School of Veterinary Medicine, State University of Ceará (Universidade Estadual do Ceará, UECE), 1700 Doutor Silas Munguba Avenue, Fortaleza, 0714-903 CE, Brazil., Marinari P; Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, VA 22630, USA., Comizzoli P; Smithsonian Conservation Biology Institute, National Zoological Park, Washington, DC 20008, USA.
المصدر: Animals : an open access journal from MDPI [Animals (Basel)] 2020 Oct 13; Vol. 10 (10). Date of Electronic Publication: 2020 Oct 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011-
مستخلص: Systematic cryo-banking of semen and testicular tissues is critical to preserve the genetic value of recently deceased or neutered black-footed ferrets (BFFs). Specifically, recovering or producing mature sperm cells from vitrified-warmed issues offers additional options in assisted reproduction. This could, in turn, enhance the genetic management of this rare and endangered species over multiple generations. The objective of the study was to evaluate structural properties, DNA fragmentation, cell viability, and germ cell composition in vitrified testicular tissues from BFFs directly after warming or after warming plus a short in vitro culture period. Tissue biopsies from five adult BFFs were either kept fresh or vitrified with a standard protocol (using dimethylsulphoxide (DMSO) and glycerol) and warmed at 50 °C for 5 s. Some of the warmed samples were then cultured in vitro for 24 h. Fresh, warmed, and warmed/cultured tissues were analyzed using different indicators: histology of seminiferous tubules, intact Sertoli cells (vimentin labeling), DNA integrity, cell viability, germ cell composition (Oct4 and Boule labeling). Percentages of intact seminiferous tubules decreased after vitrification/warming and returned to the level of fresh samples after culture. While percentages of cells labeled with vimentin, with intact DNA integrity, or proportions of viable cells were affected by vitrification/warming, they all reached similar or better levels than the fresh tissue after culture. Proportions of cells labeled with Boule antibodies also improved during in vitro culture post-warming. We demonstrated for the first time that BFF testes subjected to vitrification, rapid warming, and short in vitro culture were viable and maintained the ability to resume germ cell progression. Cryopreserved testicular tissues could potentially contribute to new strategies to enhance BFF assisted reproduction as well as conservation efforts.
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معلومات مُعتمدة: N/A Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq; N/A Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
فهرسة مساهمة: Keywords: black-footed ferret; cryopreservation; endangered species; spermatogenesis; testicular tissue
تواريخ الأحداث: Date Created: 20201017 Latest Revision: 20201101
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7602014
DOI: 10.3390/ani10101865
PMID: 33066219
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2615
DOI:10.3390/ani10101865