دورية أكاديمية
أعرض في EDS

Lung megakaryocytes are immune modulatory cells.

التفاصيل البيبلوغرافية
العنوان: Lung megakaryocytes are immune modulatory cells.
المؤلفون: Pariser DN; Aab Cardiovascular Research Institute and.; Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Hilt ZT; Aab Cardiovascular Research Institute and., Ture SK; Aab Cardiovascular Research Institute and., Blick-Nitko SK; Aab Cardiovascular Research Institute and., Looney MR; Department of Medicine, UCSF, San Francisco, California, USA., Cleary SJ; Department of Medicine, UCSF, San Francisco, California, USA., Roman-Pagan E; Aab Cardiovascular Research Institute and., Saunders J 2nd; Center for Pediatric Biomedical Research, Department of Pediatrics, and., Georas SN; Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Veazey J; Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Madere F; Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Santos LT; Cardiovascular Research Department, University Hospital Ramón y Cajal Biotechnology, Medicine and Health Sciences PhD Program, University Francisco de Vitoria, Madrid, Spain., Arne A; Aab Cardiovascular Research Institute and., Huynh NP; Genomics Research Center, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.; Center for Translational Neuromedicine, University of Copenhagen, Copenhagen, Denmark., Livada AC; Aab Cardiovascular Research Institute and.; Department of Pathology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Guerrero-Martin SM; Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Lyons C; Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Metcalf-Pate KA; Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., McGrath KE; Center for Pediatric Biomedical Research, Department of Pediatrics, and., Palis J; Center for Pediatric Biomedical Research, Department of Pediatrics, and., Morrell CN; Aab Cardiovascular Research Institute and.; Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.; Department of Pathology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
المصدر: The Journal of clinical investigation [J Clin Invest] 2021 Jan 04; Vol. 131 (1).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 7802877 Publication Model: Print Cited Medium: Internet ISSN: 1558-8238 (Electronic) Linking ISSN: 00219738 NLM ISO Abbreviation: J Clin Invest Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : Ann Arbor, MI : American Society for Clinical Investigation
Original Publication: New Haven [etc.] American Society for Clinical Investigation.
مواضيع طبية MeSH: Antigen-Presenting Cells/*immunology , Lung/*immunology , Megakaryocytes/*immunology, Animals ; CD4-Positive T-Lymphocytes/immunology ; Histocompatibility Antigens Class II/genetics ; Histocompatibility Antigens Class II/immunology ; Lymphocyte Activation ; Mice ; Mice, Knockout ; RNA-Seq ; Single-Cell Analysis
مستخلص: Although platelets are the cellular mediators of thrombosis, they are also immune cells. Platelets interact both directly and indirectly with immune cells, impacting their activation and differentiation, as well as all phases of the immune response. Megakaryocytes (Mks) are the cell source of circulating platelets, and until recently Mks were typically only considered bone marrow-resident (BM-resident) cells. However, platelet-producing Mks also reside in the lung, and lung Mks express greater levels of immune molecules compared with BM Mks. We therefore sought to define the immune functions of lung Mks. Using single-cell RNA sequencing of BM and lung myeloid-enriched cells, we found that lung Mks, which we term MkL, had gene expression patterns that are similar to antigen-presenting cells. This was confirmed using imaging and conventional flow cytometry. The immune phenotype of Mks was plastic and driven by the tissue immune environment, as evidenced by BM Mks having an MkL-like phenotype under the influence of pathogen receptor challenge and lung-associated immune molecules, such as IL-33. Our in vitro and in vivo assays demonstrated that MkL internalized and processed both antigenic proteins and bacterial pathogens. Furthermore, MkL induced CD4+ T cell activation in an MHC II-dependent manner both in vitro and in vivo. These data indicated that MkL had key immune regulatory roles dictated in part by the tissue environment.
التعليقات: Comment in: J Clin Invest. 2021 Jan 4;131(1):. (PMID: 33393510)
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معلومات مُعتمدة: R21 HL153409 United States HL NHLBI NIH HHS; R01 HL141106 United States HL NHLBI NIH HHS; R01 AI144241 United States AI NIAID NIH HHS; R01 HL107386 United States HL NHLBI NIH HHS; F31 HL147458 United States HL NHLBI NIH HHS; F31 HL145922 United States HL NHLBI NIH HHS; R01 HL142152 United States HL NHLBI NIH HHS; T32 OD011089 United States OD NIH HHS; T32 HL066988 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Hematology; Inflammation; Platelets
المشرفين على المادة: 0 (Histocompatibility Antigens Class II)
تواريخ الأحداث: Date Created: 20201020 Date Completed: 20210907 Latest Revision: 20210907
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7773372
DOI: 10.1172/JCI137377
PMID: 33079726
قاعدة البيانات: MEDLINE
الوصف
تدمد:1558-8238
DOI:10.1172/JCI137377