دورية أكاديمية
أعرض في EDS

Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library.

التفاصيل البيبلوغرافية
العنوان: Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library.
المؤلفون: Gittens RA; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Centro de Neurociencias, INDICASAT AIP, Panama, Republic of Panama., Almanza A; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama., Bennett KL; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Smithsonian Tropical Research Institute, Panama, Republic of Panama., Mejía LC; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Smithsonian Tropical Research Institute, Panama, Republic of Panama., Sanchez-Galan JE; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Grupo de Investigación en Biotecnología, Bioinformática y Biología de Sistemas, Facultad de Ingeniería de Sistemas Computacionales, Universidad Tecnológica de Panamá, Panama, Republic of Panama., Merchan F; Grupo de Investigación en Sistemas de Comunicaciones Digitales Avanzados, Facultad de Ingeniería Eléctrica, Universidad Tecnológica de Panamá, Panama, Republic of Panama., Kern J; Grupo de Investigación en Sistemas de Comunicaciones Digitales Avanzados, Facultad de Ingeniería Eléctrica, Universidad Tecnológica de Panamá, Panama, Republic of Panama.; ENSEIRB-MATMECA-Bordeaux INP, France., Miller MJ; Department of Anthropology, Pennsylvania State University, University Park, PA, United States of America.; University of Alaska Museum, University of Alaska Fairbanks, Fairbanks, AK, United States of America., Esser HJ; Department of Environmental Sciences, Wageningen University, Wageningen, the Netherlands., Hwang R; Department of Biology, Swarthmore College, Swarthmore, PA, United States of America., Dong M; Department of Biology, Swarthmore College, Swarthmore, PA, United States of America., De León LF; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Department of Biology, University of Massachusetts Boston, Boston, MA, United States of America., Álvarez E; Programa Centroamericano de Maestría en Entomología, Universidad de Panamá, Panama, Republic of Panama., Loaiza JR; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama.; Smithsonian Tropical Research Institute, Panama, Republic of Panama.; Programa Centroamericano de Maestría en Entomología, Universidad de Panamá, Panama, Republic of Panama.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2020 Oct 27; Vol. 14 (10), pp. e0008849. Date of Electronic Publication: 2020 Oct 27 (Print Publication: 2020).
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Ixodidae/*chemistry , Proteomics/*methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods, Animals ; Arthropod Proteins/chemistry ; Arthropod Proteins/metabolism ; Disease Vectors/classification ; Ixodidae/classification ; Ixodidae/metabolism
مستخلص: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens.
Competing Interests: The authors have declared that no competing interests exist.
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معلومات مُعتمدة: EP-C-15-002 United States EPA EPA; EP-D-15-016 United States EPA EPA
المشرفين على المادة: 0 (Arthropod Proteins)
تواريخ الأحداث: Date Created: 20201027 Date Completed: 20210208 Latest Revision: 20240430
رمز التحديث: 20240430
مُعرف محوري في PubMed: PMC7647123
DOI: 10.1371/journal.pntd.0008849
PMID: 33108372
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0008849