دورية أكاديمية
Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine-Modulating Proteins as Therapeutics.
| العنوان: | Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine-Modulating Proteins as Therapeutics. |
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| المؤلفون: | Burgin M; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA. mburgin@asu.edu., Yaron JR; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA., Zhang L; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA., Guo Q; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.; Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Daggett J; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA., Kilbourne J; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA., Lowe KM; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA., Lucas AR; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.; Cardiology, Saint Joseph's Hospital, Dignity Health, Phoenix, AZ, USA. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2225, pp. 257-273. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Graft Survival*, Anti-Inflammatory Agents/*pharmacology , Graft Rejection/*prevention & control , Immunologic Factors/*pharmacology , Kidney Transplantation/*methods , Myxoma virus/*chemistry , Viral Proteins/*pharmacology, Animals ; Anti-Inflammatory Agents/immunology ; Anti-Inflammatory Agents/metabolism ; Biomarkers/analysis ; Chemokines/biosynthesis ; Complement C4b/genetics ; Complement C4b/immunology ; Female ; Gene Expression ; Graft Rejection/genetics ; Graft Rejection/immunology ; Graft Rejection/pathology ; Immunologic Factors/biosynthesis ; Immunologic Factors/immunology ; Kidney/immunology ; Kidney/surgery ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Peptide Fragments/genetics ; Peptide Fragments/immunology ; Receptors, Interferon/biosynthesis ; Receptors, Interferon/immunology ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/immunology ; Recombinant Proteins/pharmacology ; Transplantation, Homologous ; Viral Proteins/biosynthesis ; Viral Proteins/immunology |
| مستخلص: | Solid tissue transplant is a growing medical need that is further complicated by a limited donor organ supply. Acute and chronic rejection occurs in nearly all transplants and reduces long-term graft survival, thus increasing the need for repeat transplantation. Viruses have evolved highly adapted responses designed to evade the host's immune defenses. Immunomodulatory proteins derived from viruses represent a novel class of potential therapeutics that are under investigation as biologics to attenuate immune-mediated rejection and damage. These immune-modulating proteins have the potential to reduce the need for traditional posttransplant immune suppressants and improve graft survival. The myxoma virus-derived protein M-T7 is a promising biologic that targets chemokine and glycosaminoglycan pathways central to kidney transplant rejection. Orthotopic transplantations in mice are prohibitively difficult and costly and require a highly trained microsurgeon to successfully perform the procedure. Here we describe a kidney-to-kidney subcapsular transplant model as a practical and simple method for studying transplant rejection, a model that requires fewer mice. One kidney can be used as a donor for transplants into six or more recipient mice. Using this model there is lower morbidity, pain, and mortality for the mice. Subcapsular kidney transplantation provides a first step approach to testing virus-derived proteins as new potential immune-modulating therapeutics to reduce transplant rejection and inflammation. |
| فهرسة مساهمة: | Keywords: Immunomodulatory; Rejection; Renal; Therapeutics; Transplant; Viral protein |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Biomarkers) 0 (Chemokines) 0 (Immunologic Factors) 0 (M-T7 protein, Myxoma virus) 0 (Peptide Fragments) 0 (Receptors, Interferon) 0 (Recombinant Proteins) 0 (Viral Proteins) 80295-50-7 (Complement C4b) 80295-52-9 (complement C4d) |
| تواريخ الأحداث: | Date Created: 20201027 Date Completed: 20210326 Latest Revision: 20210326 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-0716-1012-1_15 |
| PMID: | 33108668 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-1012-1_15 |