دورية أكاديمية
أعرض في EDS

Ribosome Fingerprinting with a Solid-State Nanopore.

التفاصيل البيبلوغرافية
العنوان: Ribosome Fingerprinting with a Solid-State Nanopore.
المؤلفون: Raveendran M; School of Electronic and Electrical Engineering and Pollard Institute, University of Leeds, Leeds LS2 9JT, U.K., Leach AR; School of Electronic and Electrical Engineering and Pollard Institute, University of Leeds, Leeds LS2 9JT, U.K., Hopes T; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, U.K.; LeedsOmics, University of Leeds, Leeds LS2 9JT, U.K., Aspden JL; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, U.K.; LeedsOmics, University of Leeds, Leeds LS2 9JT, U.K., Actis P; School of Electronic and Electrical Engineering and Pollard Institute, University of Leeds, Leeds LS2 9JT, U.K.; LeedsOmics, University of Leeds, Leeds LS2 9JT, U.K.; Bragg Centre for Materials Research, Leeds LS2 9JT, U.K.
المصدر: ACS sensors [ACS Sens] 2020 Nov 25; Vol. 5 (11), pp. 3533-3539. Date of Electronic Publication: 2020 Oct 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Chemical Society, [2016]-
مواضيع طبية MeSH: Nanopores*, Humans ; Polyribosomes/metabolism ; Protein Biosynthesis ; RNA, Messenger/metabolism ; Ribosomes/metabolism
مستخلص: Nanopores hold great potential for the analysis of complex biological molecules at the single-entity level. One particularly interesting macromolecular machine is the ribosome, responsible for translating mRNAs into proteins. In this study, we use a solid-state nanopore to fingerprint 80S ribosomes and polysomes from a human neuronal cell line and Drosophila melanogaster cultured cells and ovaries. Specifically, we show that the peak amplitude and dwell time characteristics of 80S ribosomes are distinct from polysomes and can be used to discriminate ribosomes from polysomes in mixed samples. Moreover, we are able to distinguish large polysomes, containing more than seven ribosomes, from those containing two to three ribosomes, and demonstrate a correlation between polysome size and peak amplitude. This study highlights the application of solid-state nanopores as a rapid analytical tool for the detection and characterization of ribosomal complexes.
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; BB/S007407/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 105615/Z/14/Z United Kingdom WT_ Wellcome Trust
فهرسة مساهمة: Keywords: nanopipette; nanopore; polysome; ribosome; single-molecule; translation
المشرفين على المادة: 0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20201028 Date Completed: 20210514 Latest Revision: 20210514
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7706116
DOI: 10.1021/acssensors.0c01642
PMID: 33111519
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-3694
DOI:10.1021/acssensors.0c01642