دورية أكاديمية
Pro-apoptotic properties and mitochondrial functionality in platelet-like-particles generated from low Aspirin-incubated Meg-01 cells.
| العنوان: | Pro-apoptotic properties and mitochondrial functionality in platelet-like-particles generated from low Aspirin-incubated Meg-01 cells. |
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| المؤلفون: | Freixer G; Medicine., Zekri-Nechar K; Medicine., Zamorano-León JJ; Public Health and Maternal and Child Health., Hugo-Martínez C; Medicine., Butta NV; Haematology Department, Hospital Universitario La Paz, idiPaz, Madrid, Spain., Monzón E; Haematology Department, Hospital Universitario La Paz, idiPaz, Madrid, Spain., Recio MJ; Immunology, Ophthalmology and ORL., Giner M; Surgery Departments, School of Medicine, Universidad Complutense, Madrid, Spain., López-Farré A; Medicine. |
| المصدر: | Platelets [Platelets] 2021 Nov 17; Vol. 32 (8), pp. 1063-1072. Date of Electronic Publication: 2020 Oct 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 9208117 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1369-1635 (Electronic) Linking ISSN: 09537104 NLM ISO Abbreviation: Platelets Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Edinburgh ; New York : Churchill Livingstone, c1990- |
| مواضيع طبية MeSH: | Apoptosis/*drug effects , Aspirin/*therapeutic use , Blood Platelets/*drug effects , Membrane Potential, Mitochondrial/*immunology, Aspirin/pharmacology ; Humans |
| مستخلص: | Long-term therapy with low Aspirin (ASA) dose is basis to prevent thrombotic acute events. However, the anti-platelet mechanisms of ASA remain not completely known. The aim was to analyze if in vitro exposure of human megakaryocytes to low ASA concentration may alter the apoptotic features of the newly formed platelets. Cultured Meg-01 cells, a human megakaryoblastic cell line, were stimulated to form platelets with 10 nmol/L phorbol 12-myristate-13-acetate (PMA) in the presence and absence of ASA (0.33 mmol/L). Results revealed that platelet-like particles (PLPs) derived from ASA-exposed Meg-01 cells, showed higher content of pro-apoptotic proteins Bax and Bak than PLPs from non-ASA incubated Meg-01 cells. It was accompanied of reduced cytochrome C oxidase activity and higher mitochondrial content of PTEN-induced putative kinase-1 in PLPs from ASA-incubated Meg-01 cells. However, only after calcium ionophore A23187 stimulation, caspase-3 activity, the cytosolic cytochrome C content, and reduction of mitochondrial membrane potential were higher in PLPs from ASA-incubated megakaryocytes than in those from Meg-01 without ASA. Nitric oxide synthase 3 content was higher in PLPs from ASA-exposed Meg-01 cells than in PLPs from non-ASA incubated Meg-01 cells. The L-arginine antagonist, NG-Nitro-L-arginine Methyl Ester, reduced caspase-3 activity in A23187-stimulated PLPs generated from ASA-incubated Meg-01 cells. As conclusions exposure of megakaryocyte to ASA promotes that the newly generated PLPs have, under stimulating condition, higher sensitivity to go into apoptosis than those PLPs generated from Meg-01 cells without ASA. It could be associated with differences in mitochondrial functionality and NO formation. |
| فهرسة مساهمة: | Keywords: Apoptosis; Aspirin; Megakaryocytes; Mitochondria; Nitric Oxide; Platelets |
| المشرفين على المادة: | R16CO5Y76E (Aspirin) |
| تواريخ الأحداث: | Date Created: 20201028 Date Completed: 20220217 Latest Revision: 20220217 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1080/09537104.2020.1839637 |
| PMID: | 33111589 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1369-1635 |
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| DOI: | 10.1080/09537104.2020.1839637 |