دورية أكاديمية
Bovine alphaherpesvirus 1 (BHV1) infection in testes and epididymis from bulls from a slaughterhouse.
| العنوان: | Bovine alphaherpesvirus 1 (BHV1) infection in testes and epididymis from bulls from a slaughterhouse. |
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| المؤلفون: | Dias Queiroz-Castro VL; Department of Veterinary, Division of Animal Reproduction, Laboratory of Oocyte Maturation and 'in vitro' Fertilization, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Santos MR; Department of Veterinary, Division of Preventive Medicine and Public Health, Laboratory of Animal Virology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Augusto de Azevedo-Júnior M; Department of Veterinary, Division of Animal Reproduction, Laboratory of Oocyte Maturation and 'in vitro' Fertilization, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Paulino da Costa E; Department of Veterinary, Division of Animal Reproduction, Laboratory of Oocyte Maturation and 'in vitro' Fertilization, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Pereira Alves SV; Department of Veterinary, Division of Animal Reproduction, Laboratory of Oocyte Maturation and 'in vitro' Fertilization, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Nascimento Silva LM; Department of Veterinary, Division of Preventive Medicine and Public Health, Laboratory of Animal Virology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Dohanik VT; Department of General Biology, Division of Structural and Cell Biology, Laboratory of Structural Biology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil., Silva-Júnior A; Department of Veterinary, Division of Preventive Medicine and Public Health, Laboratory of Animal Virology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: abelardo.junior@ufv.br. |
| المصدر: | Theriogenology [Theriogenology] 2021 Jan 01; Vol. 159, pp. 1-6. Date of Electronic Publication: 2020 Oct 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Epididymis* , Herpesvirus 1, Bovine*, Abattoirs ; Abortion, Veterinary ; Animals ; Cattle ; Female ; Male ; Pregnancy ; Testis |
| مستخلص: | Bovine alphaherpesvirus 1 (BHV1) is an agent associated with reproductive disease in cattle. Viral pathogenicity is related to disorders such as temporary infertility, embryonic death, and abortions in affected animals. Considering that natural infections in genital organs of males are understudied, this investigation evaluated the presence of BHV1 in both testicular and epididymal tissues obtained from naturally infected bulls by the evaluation of the presence of the BHV1 genome and antigens. Sixty samples of blood and genital organs of 60 bulls that were not vaccinated against BHV1 were assayed. Fragments from testes and head, body, and tail of epididymides were processed and analyzed by nested PCR and immunofluorescence with confocal laser scanning microscopy. Also, the BHV1 gB glycoprotein gene of 14 positive samples was partially sequenced. The percentage of BHV1 presence obtained by the immunolocalization assay corresponded to 95.9% of the testes, 100% of the epidydimal tissue in the head and tail portions, and 98% of the epididymal body. The nested PCR assay detected the viral nucleic acid in 59.2% of the testicular tissue and in 65.3, 75.5, and 83.7% of epididymis head, body, and tail samples, respectively. The partial sequences analyzed presented 100% of identity with other BHV1 strains. Accordingly, BHV1 detection in testes and epididymides of naturally infected bulls suggests that these organs may be sources of viral infection for semen. Competing Interests: Declaration of competing interest The authors declare that they have no competing interests. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Artificial insemination; BHV1 source; BHV1 spread; Semen contamination |
| تواريخ الأحداث: | Date Created: 20201028 Date Completed: 20210621 Latest Revision: 20210621 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.theriogenology.2020.10.001 |
| PMID: | 33113438 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3231 |
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| DOI: | 10.1016/j.theriogenology.2020.10.001 |